摘要
菰黑粉菌与茭白植株互作形成膨大肉质茎——茭白,已成为我国第二大水生蔬菜。作为一种二态性真菌,菰黑粉菌的二型态转换与侵染茭白密切相关,对茭白孕茭至关重要。本研究基于菰黑粉菌全基因组序列,采用t Blastn进行同源基因查找,克隆得到了玉米瘤黑粉菌Fuz7的同源基因UeFuz7。该基因c DNA全长1 308 bp,无内含子,编码435个氨基酸,在黑粉菌中相对保守。酵母双杂交实验发现,UeFuz7可与UeKpp2蛋白互作。同时其表达模式分析发现,在菰黑粉菌的二型态转换发生时UeFuz7的相对表达量最高。进一步通过PEG介导的原生质体转化获得UeFuz7突变体,表型分析发现:在二型态转换过程中,UeFuz7突变菌株的接合管形成和菌丝生长能力明显减弱。以上结果表明UeFuz7作用于菰黑粉菌的二型态转换。
Jiaobai,the second largest aquatic vegetable in China,was formed because of the interaction of Ustilago esculenta and its host Zizania latifolia.As a dimorphic fungus,the dimorphic transition of U.esculenta is closely related to its early infection,which is very important to formation of Jiaobai.In this study,the Ustilago maydis Fuz7 homologous gene UeFuz7 in U.esculenta was cloned,through homologous gene search by t Blastn based on the whole genome sequence of U.esculenta.The c DNA of UeFuz7 is 1 308 bp,without introns,encoding 435 amino acids,which is relatively conservative in the smut fungi.It was found that UeFuz7 interacted with UeKpp2 by yeast double hybrid experiment.Besides,its expression pattern analysis showed that the relative expression of UeFuz7 was the highest during the dimorphic transition of U.esculenta.Furthermore,PEG mediated protoplast transformation was carried out to construct UeFuz7 mutant,and phenotypic analysis found that UeFuz7 disrupted mutants significantly reduced the ability of conjugation tube formation and hyphal growth during dimorphic transition.The above results indicate that UeFuz7 functions on the dimorphic transition of U.esculenta.
作者
刘洪磊
于金梦
曹乾超
陈彬倩
陆朝晶
叶子弘
俞晓平
张雅芬
LIU Hong-lei;YU Jin-meng;CAO Qian-chao;CHEN Bin-qian;LU Chao-jing;YE Zi-hong;YU Xiao-ping;ZHANG Ya-fen(Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine,College of Life Sciences,China Jiliang University,Hangzhou 310018,China;Microbiological Inspection Center,Sichuan Institute for Food and Drug Control,Chengdu 611731,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2019年第2期203-211,共9页
Acta Phytopathologica Sinica
基金
国家自然科学基金项目(31600634
31770828)
