摘要
建立超高效液相色谱法(UPLC),测定桦褐孔菌中三种主要化合物原儿茶醛、丁香酸和(E)-4-(3,4-二羟基苯基)丁-3-烯-2-酮含量。采用UPLC法,色谱柱为CORTECS UPLC T3(2. 1mm×100mm,1. 6μm),流动相为乙腈—水(含0. 1%甲酸),柱温为40℃,流速为0. 4mL/min,进样量为1μL,检测波长为280nm。结果表明:原儿茶醛、丁香酸和(E)-4-(3,4-二羟基苯基)丁-3-烯-2-酮分别在0. 1953125—6. 25μg/m L(R2=1. 0000)、0. 1953125—6. 25μg/m L (R2=0.9998)、0. 1953125—6. 25μg/m L(R2=1. 0000)范围内线性关系良好,平均回收率(n=9)分别为98. 150%(RSD=1. 924%)、95. 787%(RSD=2. 487%)、100. 324%(RSD=1. 541%)。不同产地间的桦褐孔菌含量测定结果表明,三种化合物含量的差异较大,吉林长白山最高,俄罗斯西伯利亚次之,黑龙江大兴安岭最低。该方法操作简便、结果准确可靠、重现性好,可为桦褐孔菌的定量分析和质量评价提供科学的依据。
To establish an Ultra High Performance Liquid Chromatography( UPLC) method for simultaneous determination of Protocatechualdehyde,Syringic acid,( E)-4-( 3,4-dihydroxyphenyl) but-3-en-2-one of Inonotus obliquus( Fr.) Pilat. An UPLC method was carried out on a CORTECS UPLC T3 chromatographic column( 2. 1 mm × 100 mm,1. 6μm) at 40℃. The mobile phase was acetonitrile-water( containing 0. 1% formic acid). The flow rate was 0. 4 m L/min,the injection volume was 1 μL and the detection wavelength was 280 nm. The results showed: The calibration curve were linear within the range of 0. 1953125—6. 25 μg/m L( R2= 1.0000) for Protocatechualdehyde,0. 1953125—6. 25μg/m L( R2= 0. 9998) for Syringic acid,0. 1953125—6. 25 μg/m L( R2= 1. 0000)for( E)-4-( 3,4-dihydroxyphenyl) but-3-en-2-one,and the average recovery rate( n = 9) were 98. 150%( RSD = 1.924%),95. 787%( RSD = 2. 487%),100. 324%( RSD = 1. 541%),respectively. The results of different habitats showed that the content of three compounds varied greatly,the highest in Changbai Mountain,Jilin,followed by Siberia in Russia and the lowest in Daxinganling,Heilongjiang. Conclusion: The method was simple,accurate,reliable and reproducible,which provided scientific basis for the quantitative analysis and quality evaluation of Inonotus obliquus( Fr.) Pilat.
作者
张士彦
卢秋霞
贺丽波
孙意冉
罗朝梅
李玉
黄磊
唐琳
ZHANG Shi-yan;LU Qiu-xia;HE Li-bo;SUN Yi-ran;LUO Chao-mei;LI Yu;HUANG Lei;TANG Lin(Sichuan University College of Life Sciences;Sichuan University Institute of New Energy and Low-carbon Technology,Chengdu 610000,China)
出处
《资源开发与市场》
CAS
2019年第4期543-546,共4页
Resource Development & Market
基金
国家自然科学基金项目(编号:31570351)
