摘要
根据猪链球菌2型的溶血素(haemolysin)的基因序列设计一对引物,成功地扩增了溶血素基因,并建立了检测溶血素的PCR方法.用EcoR 限制性内切酶进行酶切,获得了与预期大小一致的869bp和633bp的两个片段.同时另设计一对内引物,进行巢式PCR,亦能扩增出预期片段.PCR检测的敏感程度可达100个细菌;对马链球菌兽疫亚种、猪丹毒杆菌和猪放线杆菌等的PCR检测结果均呈阴性.表明建立的猪链球菌2型溶血素PCR检测方法,其特异性和敏感性均很高,可作为猪链球菌病快速诊断的方法.
A PCR assay for rapid and sensitive detection of haemolysin of Streptococcus suis type 2 was established. The PCR primers based on the haemolysin of S. suis type 2 succeeded in amplifying a 1502 bp PCR product. The PCR product could be digested into two fragments of 869 bp and 633 bp by (EcoRⅠ.) With the nested PCR, a 1443 bp fragment was produced. No PCR product was extended from Streptococcus equi subsp.zooepidemicus, Erysipelothrix rhusiopathial and Actinobacillus. suis. The PCR technique could detect as low as 100 bacterial cells. The results show that haemolysin-based PCR is a highly specific and sensitive diagnostic tool for detection of S. suis type 2.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2004年第1期78-82,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省科委重点资助项目(00112226).
