摘要
目的:研究大蒜Allium sativum地上部分正丁醇萃取部位的化学成分。方法:大蒜地上部分用70%乙醇加热回流提取,合并提取液减压回收溶剂得总提取物,总提取物稠膏以适量蒸馏水混悬,依次用石油醚、乙酸乙酯及水饱和正丁醇萃取,得石油醚萃取部位、乙酸乙酯萃取部位和正丁醇萃取部位,对正丁醇萃取部位采用正相硅胶柱色谱,ODS柱色谱及制备HPLC等手段进行分离纯化,并根据所得化合物的理化性质和1H-NMR,13C-NMR,DEPT,HMBC及HR-ESI-MS等波谱数据鉴定其结构。结果:从大蒜地上部分正丁醇萃取部位分离得到7个化合物,分别鉴定为2-甲氧基-苯酚-1-O-α-L-吡喃阿拉伯糖基-(1→6)-β-D-吡喃葡萄糖苷(1),苯乙醇-4-O-β-D-木糖基-(1→6)-β-D-葡萄糖苷(2),反式白藜芦醇3-O-β-D-吡喃葡萄糖苷(3),豆甾醇(4),β-谷甾醇(5),豆甾醇-3-O-β-D-吡喃葡萄糖苷(6),胡萝卜苷(7)。结论:化合物1属于酚苷类的新化合物,并将其命名为大蒜新苷J;化合物2~7属于首次从该植物中发现。
Objective:To study the chemical constituents from the aerial part of Allium sativum.Method:The aerial part of A.sativum was treated by heating reflux extraction with 70%ethanol.The combined extracting solution was treated with decompressed recovery solvent to obtain the total extract.The total extract’s thick paste was suspended with an appropriate amount of distilled water,and then extracted with petroleum ether,ethyl acetate and water-saturated n-butyl alcohol to obtain petroleum ether extraction fraction,ethyl acetate extraction fraction and n-butyl alcohol extraction fraction.The n-butyl alcohol extraction fraction was isolated and purified by positive phase silica gel column chromatography,ODS column chromatography and preparative HPLC,etc.The structures of the chemical constituents were elucidated by means of physicochemical properties and spectroscopic analysis,and spectral data,like1H-NMR,13C-NMR,DEPT,HMBC and HR-ESI-MS.Result:Seven compounds were isolated and identified as 2-methoxy-phenol-1-O-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranoside(1),phenyl-ethanol-4-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside(2),transresveratrol 3-O-β-D-glucopyranoside(3),stigmasterol(4),β-sitosterol(5),stigmasterol-3-O-β-D-glucopyranoside(6)and daucosterol(7).Conclusion:Compound 1 is a new phenolic glycoside named dasuanxinoside J,and compounds 2-7 are isolated from A.sativum for the first time.
作者
胡斌
匡海学
辛运杰
王秋红
HU Bin;KUANG Hai-xue;XIN Yun-jie;WANG Qiu-hong(Heilongjiang Key Laboratory of Traditional Chinese Medicine(TCM)and Natural Medicine Pharmacodynamic Material Basis,Key Laboratory of Basis and Application Research of Northern Medicine under Ministry of Education,Heilongjiang University of Chinese Medicine,Harbin150040,China;School of TCM,Guangdong Pharmaceutical University,Guangzhou510006,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第22期121-125,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点研发计划“中医药现代化研究”重点专项(2018YFC1707100)
关键词
大蒜
地上部分
酚苷类
大蒜新苷J
植物甾醇类
Allium sativum
aerial part
phenolic glycosides
dasuanxinoside J
phytosterols
