摘要
目的:制备5个产地15批次野菊花饮片标准汤剂并确立其质量评价方法,研究其出膏率、指标成分含量及转移率、指纹图谱等数据,为野菊花饮片标准汤剂及其配方颗粒的制备提供参考。方法:参照传统煎药工艺制备标准汤剂,采用超高效液相色谱-二极管阵列检测器(UPLC-DAD)测定指标成分蒙花苷的含量,计算该成分的转移率,绘制指纹图谱,采用真空干燥法制备浸膏粉,计算出膏率。结果:15批野菊花饮片标准汤剂中蒙花苷质量浓度0. 19～0. 74 g·L^(-1),转移率21. 95%～66. 23%,平均转移率37. 12%(RSD 11. 8%); p H 5. 1～5. 5;出膏率24. 7%～32. 5%,平均出膏率27. 87%(RSD 2. 4%)。指纹图谱共有峰有9个,确认其中2个色谱峰(2号和9号)分别为绿原酸和蒙花苷。结论:建立的制备工艺符合传统汤剂制备方法且稳定可行,可用于野菊花饮片标准汤剂的制备及质量评价。
Objective: To study the dry extract rate, determination and transfer rate of maker compounds,fingerprint and others of standard decoction of Chrysanthemi Indici Flos and provide basic data for the preparation of this standard decoction and its dispensing granules by establishing 15 batches of standard decoction of Chrysanthemi Indici Flos from 5 different places. Method: The standard decoction of Chrysanthemi Indici Flos was prepared based on the traditional decoction process,the content of linarin was determined by UPLC-DAD,the transfer rate of this composition was calculated,the fingerprint was drawn,the extract powder was prepared by vacuum drying,and the dry extract rate was calculated. Result: The concentration of linarin in 15 batches of standard decoction of Chrysanthemi Indici Flos was 0. 19-0. 74 g·L-1,the transfer rate of linarin was 21. 95%-66. 23%,its average transfer rate was 37. 12% with RSD of 11. 8%,the pH value was 5. 1-5. 5,the range of dry extract rate was 24. 7%-32. 5%,the average dry extract rate was 27. 87% with RSD of 2. 4%. There were 9 major common peaks in the fingerprint and 2 peaks( No. 2 and No. 9) were confirmed,such as chlorogenic acid and linarin. Conclusion: The preparation method in this research conforms to the traditional decoction method and is stable and feasible. It can be used for the preparation and quality evaluation of standard decoction of Chrysanthemi Indici Flos.
作者
郭杰
孙博
朱广伟
李西文
张鹏
李雅静
杉田亨
友田健久
李琦
GUO Jie;SUN Bo;ZHU Guang-wei;LI Xi-wen;ZHANG Peng;LI Ya-jing;TORU Sugita;TAKEHISA Tomoda;LI Qi(Tianjin University of Traditional Chinese Medicine(TCM),Tianjin 301617,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Harbin University of Commerce,Harbin 150076,China;Beijing University of Chinese Medicine,Beijing 100029,China;Tsumura&Co.,Tokyo 107-8521,Japan;Shanghai TCM Co.Ltd.,Shanghai 200002,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第6期144-149,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国中医科学院中药研究所应用研究项目(H2016021-06)
关键词
野菊花标准汤剂
标准煎液
指纹图谱
配方颗粒
经典名方
蒙花苷
绿原酸
standard decoction of Chrysanthemi Indici Flos
standard decoction
fingerprint
dispensing granules
classic formula
linarin
chlorogenic acid
