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荧光原位杂交技术在上尿路上皮细胞癌诊断中的应用 被引量:1

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摘要 目的探讨荧光原位杂交技术(FISH)在上尿路上皮细胞癌诊断中的可行性。方法随机留取40例正常人群及56例疑似上尿路上皮细胞癌的肉眼血尿患者的新鲜尿液。两组同时行FISH检测和尿液脱落细胞学分析,FISH检测使用荧光标记DNA探针混合物与细胞核上3、7、17号染色体着丝粒及9p16位点杂交,并通过病理检查结果进行比较。结果 56例行FISH检测的患者,通过输尿管镜活检或手术后病理检查明确诊断为上尿路上皮细胞癌患者51例,其敏感性为91.1%,特异性为92.0%。尿脱落细胞学检测为上尿路尿路上皮细胞癌患者19例,其敏感性为33.9%,特异性为94.0%,FISH与其相比,敏感性有差异性(P<0.05),特异性无差异性(P>0.05)。结论 FISH的检测为上尿路尿路上皮细胞癌的早期诊断提供了一种快速、安全、灵敏度较高的非侵入性检测方法。
出处 《中国老年学杂志》 CAS CSCD 北大核心 2015年第16期4637-4639,4639,共3页 Chinese Journal of Gerontology
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