摘要
目的通过构建针对窖蛋白(CAV)-1基因的siRNA,研究RNAi技术对MCF-7细胞cav-1基因表达的影响。方法构建siRNA表达载体p Genesil-1-sh RNA-cav-1,通过脂质体法转染到MCF-7细胞,RT-PCR法检测cav-1的m RNA表达。结果成功构建重组表达载体p Genesil-1-sh RNA-cav-1;RT-PCR检测显示转染重组质粒的乳腺癌细胞中cav-1 m RNA表达明显低于阴性对照、空白对照和正常细胞组。结论重组表达载体p Genesil-1-sh RNA-cav-1能有效抑制人乳腺癌细胞cav-1基因的表达。
Objective To construct a new siRNA targeting to cav-1 applied to regulate the expression of cav-1 of MCF-7 cell lines.Methods The expression carrier p Genesil-1-sh RNA-cav-1 was constructed,then the carrier was transfected into MCF-7 cell lines. The expression of cav-1 m RNA was detected by reverse transcription PCR. Results It was successful to contruct the recombinated expression carrier p Genesil-1-sh RNA-cav-1. The expression of cav-1 m RNA in breast cells transfected recombination plasmids was obviously lower than the one in negative control,blank control and normal cells. Conclusions Recombinated carrier p Genesil-1-sh RNA-cav-1 could effectively inhibit the expression of cav-1 in human breast cancer cell lines.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2015年第12期3194-3196,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金资助项目(81070428)
黑龙江省教育厅科学技术研究面上项目(12521533)
黑龙江省卫生厅项目(2009-336)
佳木斯大学研究生科技创新理科重点项目(LZZ2014_006)
佳木斯大学校级交叉学科研究项目(jc2014-001)
