摘要
目的利用正交试验研究铁线透骨草中槲皮素的最佳提取条件,测定不同批次铁线透骨草中槲皮素的含量。方法设置L9(34)正交试验,安排提取温度、溶媒(甲醇)量、提取时间、加盐酸量4个因素,HPLC法检测;色谱柱:Hypersil C18色谱柱(4.6mm×250mm,5μm);流动相:甲醇-0.1%乙酸(50∶50);流速:1.0mL·min-1;柱温:25℃;检测波长:375nm。结果最佳提取条件为:样品量约2.0g,加入35mL甲醇、10mL浓盐酸,浸泡0.5h,于40℃超声震荡提取40min。在此条件下测定的8个批次铁线透骨草中槲皮素的含量为0.109 9~0.880 3mg·g-1。结论提取方法效率高、时间短、温度低,是提取铁线透骨草中槲皮素较好的方法。不同批次铁线透骨草中槲皮素的含量相差较大。
Objective To study the optimal extraction conditions of quercetin from Clematis intricata Bunge by orthogonal test and determine contents of quercetin in different batches of Clematis intricata Bunge .Methods Setting the L9 (34 )orthogonal test table,arranging four factors:extraction tempera-ture,amount of solvent (methanol), extraction time and amount of hydrochloric acid.Detecting by HPLC;column:Hypersil C18 (5μm,4.6 mm × 250 mm),mobile phase:CH 3 OH-0.1% CH 3 COOH (50 ∶ 50),flow rate: 1.0 mL/min, column temperature: 25 ℃; determining wavelength: 375 nm. Results The optimal condition was that the sample with about 2.0 g conical flask with plug was poured 35 mL CH3 OH and 10 mL concentrated hydrochloric acid,then was soaked about 0.5 h,and extraced 40 min at the temperature of 40 ℃ with ultrasound concussion.Conclusion The extraction method could have high efficiency,short time and low temperature,and be better to extract quercetin from Clematis Intricata Bunge .The contents of quercetin could differ significantly in different batches of Clematis Intri-cata Bunge .
出处
《河北北方学院学报（自然科学版）》
2015年第2期13-16,共4页
Journal of Hebei North University:Natural Science Edition
基金
河北省中药局资助项目(2013077)
河北北方学院应用化学创新团队(CXTD1306)
