摘要
目的本研究观察乌司他丁对中度烧冲复合伤大鼠肺组织炎症介质、肺微血管通透性和肺组织含水率的影响。方法将80只SD雄性大鼠随机分为对照组(n=40)和乌司他丁组(n=40),两组均造成25%总体表面积深Ⅱ度烧伤+中度冲击伤的中度烧冲复合伤模型。于伤后即刻分别静脉注射0.9%氯化钠溶液和乌司他丁40 000 U/kg 1 m L。分别于伤后6、24 h采用酶联免疫吸附试验(ELISA)检测2组大鼠肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的含量,伊文斯蓝法(EB)测定肺微血管通透性,干/湿重法测定肺组织含水率,组间计量数据行t检验。结果伤后6 h与对照组TNF-α(2912±184)pg/m L、IL-6(2793±223)pg/m L和IL-8(2178±145)pg/m L水平比较,乌司他丁组肺组织TNF-α(2103±168)pg/m L、IL-6(2315±185)pg/m L和IL-8(1827±134)pg/m L水平明显降低,差异有统计学意义(t=6.114、8.123、7.347,P<0.05);伤后24 h与肺组织TNF-α(1853±166)pg/m L、IL-6(2152±172)pg/m L和IL-8(1807±126)pg/m L水平比较,乌司他丁组TNF-α(1235±86)pg/m L、IL-6(1093±98)pg/m L和IL-8(973±77)pg/m L水平明显降低(t=5.176、7.043、5.732,P<0.05);乌司他丁组大鼠肺组织EB含量在伤后6、24 h[(63.9±3.3)、(59.8±3.2)μg/g],均明显低于对照组(78.2±3.7)、(76.3±3.5)μg/g,差异有统计学意义(t=7.023、7.382,P<0.05)。乌司他丁组大鼠肺组织含水率在伤后6、24 h[(76.3±1.45)%、(73.8±1.35)%]明显低于对照组(79.8±1.52)%、(78.3±1.47)%,差异有统计学意义(t=2.041、2.758,P<0.05)。结论乌司他丁能降低中度烧冲复合伤大鼠血清TNF-α、IL-6、IL-8炎症介质水平,抑制血管内皮细胞活化和通透性增加,对血管内皮细胞有保护作用。
Objective To investigate the effet of ulinastatin on pulmonary tissue inflammatory mediators,vistal vasopermeability and tissue water content after moderate burn-blast combined injury in rats.Methods Eighty male Sprague-Dawley rats were randomly divided into the control group and ulinastatin group. A model with moderate burn-blast combined injury,was produced in both groups. Immediately after injury rats were intravenously given either 1 m L sodium chloride solution in control group or 1 m L sodium chloride solution containing ulinastatin in ulinastatin group( 40 000 U / kg). The content of tumor necrosis factor-α,interleukin( IL)-6 and interleukin( IL)-8 were detected by enzyme linked immuno sorbent assay( ELISA) at 6 hours and 24 hours after injury. The vasopermeability was detected by the method of evans blue and the rates of tissue water content in lung were detected by the dry / wet weight. Measurement data between groups was analyzed by t test. Results Compared with control group after 6 h,the inflammatory mediators of TNF-α、IL-6 and IL-8 in ulinastatin group were( 2103 ± 168) pg / m L,( 2315 ± 185) pg / m L and( 1827 ± 134) pg / m L,significantly lower than those( 2912 ± 184) pg / m L,( 2793 ± 223) pg / m L and( 2178 ± 145) pg/m L in control group,the difference was statistically significant( t = 6. 114,8. 123,7. 347,P < 0. 05). Compared with 24 hours,the inflammatory mediators of TNF-α、IL-6 and IL-8 in ulinastatin group were( 1235 ± 86) pg / m L,( 1093 ± 98) pg / m L and( 973 ± 77) pg / m L,even more significantly lower than those( 1853 ± 166) pg / m L,( 2152 ± 172) pg / m L and( 1807 ± 126) pg / m L in control group,the difference was statistically significant( t = 5. 176,7. 043,5. 732,P < 0. 05). Compared with control group after 6 hours and 24 hours,the visceral content of evans blue in ulinastatin group were( 63. 9 ± 3. 3),( 59. 8 ± 3. 2) μg/g,significantly lower than those( 78. 2 ± 3. 7),( 76. 3 ± 3. 5) μg/g in control group,the difference was statistically significant( t = 7
出处
《中华损伤与修复杂志（电子版）》
CAS
2015年第2期132-136,共5页
Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)
基金
全军"十二五"后勤科研计划课题资助项目(CKJ12J027)
天普研究基金资助项目(UF2013270)
关键词
肺
水肿
毛细血管通透性
药物疗法
烧冲复合伤
乌司他丁
Lung
Edema
Capillary permeability
Drug therapy
Burn-blast combined injury
Ulinastatin
