摘要
[目的]探究活性乳清蛋白对化疗期间荷瘤小鼠的营养状态、免疫调节和疗效的影响。[方法 ]建立三阴乳腺癌小鼠皮下移植瘤模型,根据干预措施不同随机分为4组,分别为空白对照组(Control组)、紫杉醇化疗组(Control+紫杉醇组)、添加酪蛋白的紫杉醇化疗组(酪蛋白+紫杉醇组)、添加活性乳清蛋白的紫杉醇化疗组(活性乳清蛋白+紫杉醇组)。观察和测量小鼠体重、肿瘤体积、肿瘤重量的变化情况,比较各组小鼠的生存期,检测各组小鼠血液及肿瘤组织中谷胱甘肽(glutathione,GSH)含量。[结果]活性乳清蛋白+紫杉醇组瘤鼠体重下降速度明显缓于酪蛋白+紫杉醇组和Control+紫杉醇组,试验进行第31d活性乳清蛋白+紫杉醇组瘤鼠体重[(20.52±1.10)g],显著高于酪蛋白+紫杉醇组[(19.03±1.76)g]和Control+紫杉醇组[(18.71±0.86)g],差异具有统计学意义。活性乳清蛋白+紫杉醇组瘤鼠肿瘤体积变化与其他各组差异不具有统计学意义。活性乳清蛋白+紫杉醇组生存期较Control+紫杉醇组与酪蛋白+紫杉醇组生存期明显延长。活性乳清蛋白+紫杉醇组肿瘤组织中GSH含量[(34.5±18.0)μmol/L]低于酪蛋白+紫杉醇组[(55.3±23.8)μmol/L]和Control+紫杉醇组[(54.9±11.7)μmol/L]。而血液中,活性乳清蛋白+紫杉醇组血液的GSH含量[(19.1±0.7)μmol/L]高于酪蛋白+紫杉醇组[(13.0±8.8)μmol/L]和Control+紫杉醇组[(15.2±9.7)μmol/L]。但无论是肿瘤组织中还是血液中,各处理组间GSH含量差异均无统计学意义(P>0.05)。[结论]活性乳清蛋白联合化疗减缓小鼠体重下降,改善营养状态,抑制肿瘤增长,延长荷瘤小鼠生存期,改善预后。
[Purpose]To investigate the effect of active whey protein(ABD)on nutritional and immune status of mice bearing triple-negative breast cancer(TNBC)undergoing chemotherapy.[Methods]The triple-negative breast cancer 4T1 cells were inoculated in BALB/c mice.The tumor bearing mice were randomly divided into 4 groups with 6 mice in each group:control group,paclitaxel group,casein+paclitaxel group and ABD+paclitaxel group.The weight of mice,volume of tumor were observed and measured.The overall survival of mice and glutathione(GSH)levels in blood and tumors were analyzed.[Results]Weight of mice in ABD+paclitaxel group[(20.52±1.10)g]was significantly higher than those of casein+paclitaxel group[(19.03±1.76)g]and paclitaxel group[(18.71±0.86)g](P<0.05)on d31.The tumor sizes in ABD+paclitaxel group was lower than that in other groups(P<0.05).The overall survival of the ABD+paclitaxel group was significantly longer than paclitaxel group and casein+paclitaxel group.The GSH levels in tumor tissue of ABD+paclitaxel group,casein+paclitaxel and paclitaxel group were(34.5±18.0)μmol/L,(55.3±23.8)μmol/L and(54.9±11.7)μmol/L,respectively.However,GSH levels in blood of three groups were(19.1±0.7)μmol/L,(13.0±8.8)μmol/L and(15.2±9.7)μmol/L,respectively.There was no significant difference in GSH contents both in blood and tumor tissue among three groups(P>0.05).[Conclusion]Whey protein combined with chemotherapy can attenuate weight loss,improve nutritional status,inhibit tumor growth and prolong survival time in mice bearing triple negative breast cancer.
作者
曾珍
杜驰
涂梨
王力
王喻义
罗锋
ZENG Zhen;DU Chi;TU Li;WANG Li;WANG Yu-yi;LUO Feng(State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China;Neijiang Second People’s Hospital,Neijiang 641000,China)
出处
《中国肿瘤》
CAS
CSCD
北大核心
2019年第6期476-482,共7页
China Cancer
基金
四川省医学会青年创新课题(Q16042)
关键词
活性乳清蛋白
乳腺肿瘤
营养治疗
whey protein
breast neoplasms
nutritional therapy
