摘要
目的:检测川陈皮素对MC3T3-E1细胞增殖及成骨分化的作用及机制。方法:采用CCK-8法、碱性磷酸酶(ALP)活性检测及实时定量PCR方法评价川陈皮素对MC3T3-E1细胞增殖、成骨分化及相关基因表达的影响。结果:川陈皮素能促进MC3T3-E1的细胞增殖,刺激细胞ALP活性升高,上调BMP-2、COL-I、OCN、Runx 2的基因表达,BMP-2信号阻断剂(Noggin)能抑制成骨分化基因表达。结论:川陈皮素可促进MC3T3-E1成骨分化及成骨基因表达,BMP-2信号通路可能参与了该过程。
Objective:To investigate the effect and mechanism of nobiletin on the proliferation and osteogenic differentiation of MC3 TS-E1 cells.Methods:CCK-8 assay,alkaline phosphatase(ALP)activity assay and real-time quantitative PCR were used to evaluate the effects of nobiletin on the proliferation,osteogenesis differentiation and related gene expression of MC3 T3-E1 cells.Results:Nobiletin at concentrations of 0.1μM,1μM and 10μM concentration-dependently promoted MC3 T3-E1 proliferation,ALP activity and osteogenic gene expression,including BMP-2、COL-I、OCN、Runx2.The expression levels of gene were decreased in varying degrees after treatment with BMP-2 pathway blocker noggin.Conclusion:Nobiletin could promote osteogenic differentiation and osteogenic gene expression in MC3 T3-E1,and BMP-2 signaling pathway might be involved in this process.
作者
李强
兰庭超
卢芸
陈静
LI-Qiang;LAN Ting-chao;LU-Yun;CHEN-Jing(Department of Orthodontics,Oral Biomedical Engineering Laboratory,Shanghai Stomatological Hospital,Fudan University,Shanghai 200001,China)
出处
《中华老年口腔医学杂志》
2019年第1期9-12,共4页
Chinese Journal of Geriatric Dentistry
基金
上海市科学技术委员会资助项目(项目编号:18dz2306000)
上海市口腔病防治院基金(项目编号:SSDCZ-2016-03)
关键词
川陈皮素
成骨细胞
成骨分化
nobiletin
osteoblast
osteogenic differentiation
