摘要
【目的】烤烟育苗基质在育苗期易出现腐生真菌生长,本研究旨在了解腐生真菌的种类及其碳源代谢特征。【方法】采用组织分离培养法对腐生真菌进行分离,采用形态学和rDNA-ITS分析的方法对其进行鉴定,并利用Biolog代谢对其碳源代谢特征进行分析。【结果】共分离纯化获得3株腐生真菌,经鉴定确定菌株JZ-1和JZ-2为Trichoderma harzianum,菌株JZ-3为Trichoderma asperellum。这些腐生真菌创伤接菌时均可使烟苗发病,基质接菌时均不能使烟苗发病。它们能利用Biolog FF代谢板上44种碳源,其中赤藓糖醇、L-海藻糖、乳果糖、麦芽三糖、D-甘露糖、D-松三糖和D-山梨醇能促进其产孢。【结论】"木霉属真菌"是育苗基质上的腐生菌之一,具有较强的碳源代谢能力,研究结果对烟草育苗基质腐生真菌的认识奠定了基础。
Flue-cured tobacco seedling substrate is prone to saprophytic fungi growth. The purpose of this study is to understand the species of saprophytic fungi and their carbon source metabolism. Saprophytic fungi were isolated by tissue culture method and identi?ed by morphology and rDNA-ITS analysis. Metabolism analysis on Biolog FF metabolic plates was performed to analyze carbon source metabolism characteristics of the pathogens. Three strains of saprophytic fungi were isolated and puri?ed. Strains JZ-1 and JZ-2 were identi?ed as Trichoderma harzianum, strain JZ-3 was Trichoderma asperellum. Saprophytic fungi can infect tobacco seedlings by wounded inoculation method on tobacco leaves, while not by inoculation directly on seedling substrate. These fungi metabolized the 44 carbon sources on Biolog FF metabolic plate, among which, erythritol, L-trehalose, lactulose, maltotriose, D-mannose, D-melezitose, and D-sorbitol can promote its conidia production. It is concluded that Trichoderma is one of the saprophytic fungi existing on tobacco seedling substrate,which has strong carbon source metabolism ability. Results of this study may help to enhance our understanding of saprophytic fungi on tobacco seedling substrate.
作者
周浩
向立刚
陈乾丽
汪汉成
余知和
ZHOU Hao;XIANG Ligang;CHEN Qianli;WANG Hancheng;YU Zhihe(College of Life Science,Yangtze University,Jingzhou 434025,China;Guizhou Academy of Tobacco Sciences,Guiyang 550081,China)
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2019年第1期122-128,共7页
Acta Tabacaria Sinica
基金
中国博士后科学基金(2017M610585)
贵州省科技厅优秀青年人才培养计划(黔科合平台人才[2017]5619)
贵州省科技支撑计划(黔科合支撑[2018]2356)
中国烟草总公司贵州省公司科技项目(201711
201714)
关键词
育苗基质
腐生真菌
分离
鉴定
碳源代谢
seedling substrate
saprophytic fungi
isolation
identification
carbon source metabolism
