摘要
为调查犬圆环病毒(CanineCV)在四川部分地区的流行情况及基因组特征,本研究采用荧光定量PCR方法检测了2015年～2017年采集自四川6家宠物医院的208份腹泻犬粪便样品。结果显示CanineCV的平均阳性率为15.4%(32/208),3年间的检出率无明显变化;并且阳性样品均与一种或多种病原共感染。获得了6条完整的CanineCV Rep基因序列及1株CanineCV (CD17/2016)全基因组序列,进化分析显示CanineCV四川株(CD17/2016)与GenBank中的国内另外2株CanineCV遗传距离较远。本研究首次对国内腹泻犬进行了CanineCV感染的调查,证明CanineCV在四川部分地区宠物犬中已流行,建议把CanineCV作为犬腹泻病原诊断的一个检测内容,同时对其分子特征的研究结果将有助于进一步了解国内CanineCV的遗传多样性。
The aim of this study was to investigate the infection and the genomic characteristics of canine circovirus(CanineCV)in part areas of Sichuan during 2015 to 2017.CanineCV infection was detected in 32 diarrheic samples(15.4%)from6 pet hospitals in Sichuan by real-time PCR,there was no significant difference in the virus prevalence in different years.All CanineCV-positive samples were co-infected with enterovirus of one or more.In addition,six complete CanineCV Rep gene sequences and 1 complete genome of a CanineCV strain(CD17/2016)were sequenced.Phylogenetic analysis showed Sichuan CanineCVs were highly diverse from the other two Chinese CanineCV strains available in the GenBank.This study is the first investigation of CanineCV in dog with diarrhea symptoms in China,indicating that CanineCV is circulating in diarrheic dogs in part areas of Sichuan.This study provided the data of Chinese CanineCV genetic evolution,contributing to understanding of CanineCV genetic diversity.
作者
严楠
冯帆
李明翔
汤承
岳华
YAN Nan;FENG Fan;LI Ming-xiang;TANG Cheng;YUE Hua(College of Life Science and Technology,Southwest Minzu University,Chengdu 610041,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2019年第3期313-316,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家重点研发计划项目(2017YFD0501101)
国家民委创新团队项目(13TD0057)
西南民族大学研究生创新科研项目(CX2017SZ056)
