摘要
以感染胡葱黄条病毒(Shallot yellow stripe virus,SYSV)的大葱为材料,通过反转录聚合酶链式反应(RT-PCR)获得包含SYSV-CP全部编码序列、NIb蛋白基因5′端的部分序列和3′UTR的2个基因片段,分别命名为SYSV-CP1和SYSV-CP2,它们的编码区碱基数均为774bp,均编码258个氨基酸;在该编码区内存在2个碱基的差异,编码的蛋白存在1个氨基酸的差异。系统进化树分析表明:SYSV分离物可以区分为两个主要群体,SYSV-CP1和SYSV-CP2位于第Ⅱ组内,它们与来自浙江的SYSV的分离物亲缘关系最近,表现出一定的地理分布相关性。
Welsh onions infected by Shallot yellow stripe virus(SYSV)were taken as experiment materials.All contained SYSV-CP coding sequence,partial sequence of NIb protein gene 5'end and 2 gene fragments of 3'UTR were obtained by reverse transcription-polymerase chain reaction(RTPCR).They were named as SYSV-CP1 and SYSV-CP2,respectively.The base numbers of their coding region were both 774 bp,encoding 258 amino acids.In this coding region,2 alkali bases were different.In encoding protein,only one amino acid was different.Phylogenetic analysis showed that SYSV isolates can be divided into 2 major groups.SYSV-CP1 and SYSV-CP2 were in the second group.They had the closest relation with the isolates of SYSV from Zhejiang,representing certain geographical distribution correlation.
出处
《中国蔬菜》
北大核心
2012年第08X期36-41,共6页
China Vegetables
基金
国家自然科学基金项目(31071794)
农业部"948"计划项目(2011-Z61)
北京市农林科学院科技创新能力建设专项(KJCX201101010)
关键词
大葱
胡葱黄条病毒
外壳蛋白基因
克隆
Welsh onion
Shallot yellow stripe virus(SYSV)
Coat protein gene
Cloning
