摘要
以大头芥(红叶大头芥)为试材,研究模拟干旱、低温和强光等环境胁迫下红叶大头芥中花青素含量及其与花青素合成途径中CHI、DFR和ANS等结构基因表达的关系。首先通过同源克隆法在红叶大头芥中克隆了CHI、DFR和ANS基因,其开放阅读框分别为759、1157、1004bp,分别编码253、385、334个氨基酸。实时荧光定量RT-PCR结果表明,在环境胁迫下,红叶大头芥中花青素的积累和结构基因的大量表达需要一定处理时间的诱导。CHI基因在强光、低温和模拟干旱胁迫下的表达量无明显变化,且表达量极低;而DFR和ANS基因在低温、强光胁迫下的转录表达量随胁迫时间的延长而增加,对红叶大头芥中花青素的合成起到了重要的调控作用,且强光胁迫下DFR和ANS的表达量约为低温胁迫的两倍,推测低温和强光胁迫可能诱导了红叶大头芥中花青素合成途径不同的调控机制。
The aim of this work was to study the impact of environmental stress on big-head mustard 〔Brassica juncea(L.)Czern.et Coss.var.megarrhiza Tsen et Lee〕anthocyanins production and the expression of structural genes involved in their biosynthesis such as chalcone isomerase(CHI), dihydroflavonol 4-reductase(DFR)and anthocyanindin synthase(ANS).The CHI gene,DFR gene and ANS gene were cloned by using homology-based cloning strategy,the complementary cDNA of CHI gene has an open reading frame of 759 bp,the DFR gene is 1 157 bp,and the ANS gene is 1 004 bp.The results of real-time RT-PCR analysis suggested that the amount of anthocyanin and the expression of those structural genes required a period of time to reach the peak.The expression of CHI gene were no significant differences under strong light,low temperature and simulating drought stress.And the expression of DFR gene and ANS gene induced by low temperature and strong light stress were increased with prolonged stress time.The expression of DFR gene and ANS gene induced by low temperature and strong light stress led us to the conclusion that those two genes are important for the regulation of anthocyanin in the big-head mustard.Furthermore,the low temperature and strong light stress might induce different regulation mechanisms in the anthocyanin biosynthesis.
出处
《中国蔬菜》
北大核心
2012年第03X期27-34,共8页
China Vegetables
基金
国家自然科学基金项目(31000908)
中央高校基本科研业务费专项(XDJK2009C124)
重庆市自然科学基金重点项目(2011BA1002)
重庆市自然科学基金项目(2009BB1307)
关键词
红叶大头芥
环境胁迫
花青素
基因表达
Brassica juncea(L.)Czern.et Coss.var.megarrhiza Tsen et Lee
Environmental stress
Anthocyanin
Gene expression
