摘要
目的优选三七含量测定时供试品溶液的制备方法。方法采用HPLC法同时测定三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1,结合Box-Behnken设计-响应面法考察提取溶剂、料液比、提取温度对皂苷类成分提取工艺的影响,优选供试品溶液的制备方法。结果最佳制备方法为采用75%甲醇,回流提取1次,料液比为1∶40,提取温度为100℃。结论优选出的供试品溶液制备方法简单可行,成分提取率较高,为三七含量测定时供试品溶液制备提供参考。
Objective To study and optimize the preparation method of test solution from Panax notoginseng.Methods The effects of extraction solvent,liquid-material ratio and extraction temperature on the optimization of the preparation method of test solution of notoginsenoside and ginsenosides in P.notoginseng were investigated by Box-Behnken response surface methology.The content of notoginsenoside R1,ginsenoside Rg1,and ginsenoside Rb1 was simultaneously determined by HPLC.Results The optimum method was as follow:75%methanol was used for once reflux extraction,the ratio of liquid-material ratio was 1∶40,the extraction temperature was 100℃.Conclusion The optimum preparation of test solution is simple and feasible,and the extraction rate of components is high,which provides a reference for the preparation of test solution from P.notoginseng.
作者
梁笑
钱珊珊
孙寒
王雨宵
舒子璇
张晓倩
周卓
何霈棠
徐国兵
桂双英
LIANG Xiao;QIAN Shan-shan;SUN Han;WANG Yu-xiao;SHU Zi-xuan;ZHANG Xiao-qian;ZHOU Zhuo;HE Pei-tang;XU Guo-bing;GUI Shuang-ying(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;The Second Hospital Affiliated to Anhui University of Traditional Chinese Medicine,Hefei 230061,China;Anhui Institute for Food and Drug Control,Hefei 230051,China;Engineering Technology Research Center of Modernized Pharmaceutics,Education Office of Anhui Province,Hefei 230012,China;Anhui Key Laboratory of Traditional Chinese Medicine Compound,Hefei 230012,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第17期4145-4151,共7页
Chinese Traditional and Herbal Drugs
