摘要
目的研究神香草Hyssopus cuspidatus干燥地上部分的倍半萜类化学成分。方法采用D101大孔吸附树脂、硅胶柱色谱、ODS、Toyopearl HW-40C、Sephadex LH-20以及半制备HPLC等方法进行分离纯化,根据理化性质以及波谱数据对化合物进行结构鉴定。结果从神香草95%乙醇提取物中分离得到15个化合物,分别鉴定为3-eudesmene-1β-11-diol(1)、4-eudesmene-1β,11-diol(2)、1β,11-dihydroxy-5-eudesmene(3)、ent-4(15)-eudesmen-1α,11-diol(4)、7α,11-dihydroxy-cadin-10(14)-ene(5)、eudesmane-1β,5α,11-triol(6)、5-epi-eudesma-4(15)-ene-1β,6β-diol(7)、4(15)-eudesmene-1β,6α-diol(8)、(7R*)-opposit-4(15)-ene-1β,11-diol(9)、cryptomeridiol(10)、octahydro-4-hydroxy-3α-methyl-7-methylene-α-(1-methylethyl)-1H-indene-1-methanol(11)、caryolane-1,9β-diol(12)、(3S,5R,6S,7E)-3,5,6-trihydroxy-7-megastigmen-9-one(13)、spathulenol(14)、4β,10α-aromadendranediol(15)。结论化合物1～15均为首次从该属植物中分离得到。
Objective To study the sesquiterpenes from the aerial parts of Hyssopus cuspidatus.Methods The compounds were isolated and purified by a combination of various chromatographic techniques including D101 macroporous adsorption resin,silica gel,ODS,Toyopearl HW-40C,Sephadex LH-20,and semi-preparative HPLC.Their structures were elucidated on the basis of physicochemical properties and spectroscopic data.Results Fifteen sesquiterpenes were isolated from the 95%EtOH extracts of H.cuspidatus and their structures were elucidated as 3-eudesmene-1β-11-diol(1),4-eudesmene-1β,11-diol(2),1β,11-dihydroxy-5-eudesmene(3),ent-4(15)-eudesmen-1α,11-diol(4),7α,11-dihydroxy-cadin-10(14)-ene(5),eudesmane-1β,5α,11-triol(6),5-epieudesma-4(15)-ene-1β,6β-diol(7),4(15)-eudesmene-1β,6α-diol(8),(7R*)-opposit-4(15)-ene-1β,11-diol(9),cryptomeridiol(10),octahydro-4-hydroxy-3α-methyl-7-methylene-α-(1-methylethyl)-1H-indene-1-methanol(11),caryolane-1,9β-diol(12),(3S,5R,6S,7E)-3,5,6-trihydroxy-7-megastigmen-9-one(13),spathulenol(14),and 4β,10α-aromadendranediol(15),respectively.Conclusion Compounds 1—15 are isolated from the plants of Hyssopus genus for the first time.
作者
刘丹
朱小涛
向槿
阮长磊
尹春萍
刘惠丽
徐英黔
胡君一
尹强
方进波
LIU Dan;ZHU Xiao-tao;XIANG Jin;RUAN Chang-lei;YIN Chun-ping;LIU Hui-li;XU Ying-qian;HU Jun-yi;YIN Qiang;FANG Jin-bo(Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;School of Chemical Engineering,University of Science and Technology Liaoning,Anshan 114051,China;Wuhan Institute of Physics and Mathematics of Chinese Academy of Sciences,Wuhan 430071,China;Xinjiang Uygur Pharmaceutical Co.,Ltd.,Urumqi 830026,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第5期1049-1054,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金项目(31000150)
中央高校基本科研业务费项目(2016YXMS145)
波谱与原子分子物理国家重点实验室开放课题资助项目(T152602)
