摘要
目的 :观察AD对异常磷酸化tau蛋白表达的影响。方法 :本研究采用Aβ2 5～ 3 5片段单侧杏仁核注射 ,建立SD大鼠痴呆及记忆障碍动物模型。通过Morris水迷宫试验、放射免疫法、免疫组化法等观测大鼠空间学习记忆能力、胆碱能系统、tau蛋白异常磷酸化的改变。结果 :模型大鼠的空间学习记忆能力明显障碍 ;ChAT活性及M受体Rt值均显著降低 ;海马及额皮层异常磷酸化tau蛋白AT8表达显著增高。结论 :Aβ杏仁核单侧注射可诱导大鼠空间学习记忆障碍及胆碱能系统功能损害 ,同时伴有皮层、海马部位tau蛋白的异常磷酸化。AB是AD发生。
To study the effects of amyloid-β 25～35 on proteinexpression of hyperphosphorilated tau.Methods: The experimental rat models with dementia, spatial learning and memory impairment were induced by unilateral amyloid-β 25～35 injection into the amygdata of rats. The spatial learning and memory ability and the function of cholinergic system were observed by the means of Morris water maze and radio-ligands binding assay. The protein expression of hyperphosphorilatedtau (AT8) were estimated with immunohistochemistry.Result:The spatial learningand memory ability ofthose models had been impaired remarkably, the ChAT activity and M-receptorbinding sites were decreased significantly. Compared to the control group the expression of hyperphosphorylated tau protein (AT8) in cortices and hippocampi were increased remarkably.Conclusion: Unilateral amyloid -β 25～35 injection into the amygdala of rats could induce the impairments of spatial learning and memory ability and dysfunction ofthe cholinergic system. We could also find that amyloid -β can induce tau protein hyperphosphorylation significantly in cortices and hippocampi. Amyloid- β is an important factor in generation and development of Al).
出处
《中国现代医学杂志》
CAS
CSCD
2003年第10期5-7,共3页
China Journal of Modern Medicine
基金
国家自然科学基金重点资助课题 (39830 45 0 )
