摘要
目的 :探讨老年贲门癌FasL表达上调与肿瘤浸润性淋巴细胞 (TIL)凋亡的关系。方法 :采用免疫组织化学S P法 ,检测 5 0例老年贲门癌组织中FasL表达及TIL的数量。采用脱氧核糖核酸末端转移酶介导的缺口末端标记技术 (TUNEL) ,对其中 4 0例贲门癌组织中凋亡的TIL及肿瘤细胞进行观察。结果 :5 0例贲门癌组织FasL蛋白表达程度不等。FasL表达程度高的组织TIL计数低于FasL表达低的组织 ,同时其TIL凋亡指数高于FasL表达低的组织 ,P <0 0 5 ;而贲门癌细胞的凋亡指数低于FasL表达程度低的组织 ,P <0 0 1;TIL凋亡指数与贲门癌细胞的凋亡指数呈负相关 ,γ =- 0 5 1,P <0 0 1。结论 :老年贲门腺癌有不同程度的FasL表达 ,其表达程度与TIL的浸润程度和凋亡相关 ,可能是老年贲门癌免疫逃逸的重要机制之一。
Objective To study whether the up regulation of FasL expression is associated with increased apoptosis of tumor infiltrating lymphocytes(TIL) in elderly cardiac adenocarcinomas(ECAC).Methods The expression of FasL protein and the number of TIL in 50 specimens of ECAC were detected by immunohistochemistry.By using terminal deoxynuceotidy transferase mediated dUTP nick end labeling(TUNEL),apoptotic cells were detected in situ in 50 ECAC specimens.Results The expression of FasL protein was detected in all 50 ECAC examined.In those FasL extensive expression,the number of TIL were lower than those FasL weak expression, P <0 05,and their apoptotic index(AI) of TIL was higher and the AI of tumor cell was lower than those FasL weak expression,respectively, P <0 01.The AI of TIL was reversely correlated with the AI of tumor cell,γ=-0 52, P <0 01.Conclusion These data suggest ECAC express FasL and its expression level associate with TIL infiltrating and apoptosis,and this maybe a mechanism of immune evasion in ECAC.
出处
《肿瘤防治杂志》
2003年第9期917-919,共3页
China Journal of Cancer Prevention and Treatment
基金
江苏省医学科研基金重点课题资助项目 (H2 0 0 14 7)
关键词
贲门
胃肿瘤/代谢
膜糖蛋白裳/生物台成
淋巴细胞
肿瘤浸润
细胞死亡
cardia
stomach neoplasms/metabolism
membrane glycoproteins/biosynthesis
lymphocytes,tumor infiltrating
cells death
