摘要
目的 观察关木通所致大鼠肿瘤的发生演变过程及病理学特征。方法 雄性SD大鼠随机分为两组。关木通组大鼠(n=30)给关木通浸膏水溶液间断灌胃,初始剂量相当于马兜铃酸20mg·kg^(-1)·d^(-1),连续5d;此后剂量减至相当于马兜铃酸15mg·kg^(-1)·d^(-1),隔周给药,直至第15周。对照组大鼠(n=30)给相同体积的自来水灌胃。用药后1、4、8、12和16周分别处死大鼠(每组6只),留取气管、甲状腺、心、肺、肝、脾、胰、胃、小肠、大肠、肾、输尿管、膀胱和睾丸等14个主要脏器,观察肿瘤发生。结果 关木通组大鼠除前胃和膀胱外,其它脏器未发现肿瘤。前胃:给药后4周开始出现鳞状上皮增生及非典型增生,8周起个别大鼠开始发生鳞状细胞癌,至16周时全部大鼠均发生鳞状细胞癌。膀胱:给药后8周开始出现移行上皮增生、非典型增生及移行细胞癌,至16周时全部大鼠均发生移行细胞癌。对照组大鼠各脏器均无异常发现。结论 关木通能诱发大鼠前胃及膀胱癌,此致癌作用具有时间依赖性,并可能具有器官特异性。
Objective To observe the oncogenetic process and pathological features of tumor induced by Aristolochia manshuriensis Kom. in rats. Methods Male Sprague-Dawley rats were divided into two groups. The rats in experimental group( n = 30) received by gastric tube about 20 mg · kg-1 · d-1 aristolochic acid contained in the extract of Aristolochia manshuriensis Kom. for 5 days in the 1 st week, and then 15 mg · kg-1 · d-1 for 7 days in each group were killed at the end of 1st, 4th, 8th, 12th and 16th week, respectively. Each rat was autopsied. Ttrachea, thyroid, heart, lungs, liver, spleen, pancreas, stomach, small and large intestine, kidneys, ureters, bladder and testes were excised and fixed in 10% formalin for histological examination. Results Of the examined organs in the rats of experimental group only forestomach and bladder developed carcinomas. Forestomach: hyperplasia and atypical hyperplasia of squamous cells were found at the end of the 4th week. Squamous cell carcinoma was detected in some rats from the end of 8th week and in all rats of the 16th week. Bladder: Hyperplasia, atypical hyperplasia and carcinoma of transitional cells were found from the end of the 8th week, and this carcinoma was present in all rats at 16th week. No tumor was found in all examined organs in the rats of control group. Conclusions Aristolochia manshuriensis Kom. is a potent oncogen which can induce the squamous cell carcinoma in forestomach and the transitional cell carcinoma in bladder. Its oncogenicity is time-dependent and may be organ-specific.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2003年第4期250-253,共4页
Chinese Journal of Nephrology
基金
国家自然科学基金(30170429)
中医药管理局科研基金(2000-J-P-64)
关键词
关木通
大鼠
实验研究
致癌性
Aristolochia manshuriensis Kora
Squamous cell carcinoma
Transitional cell carcinoma
