摘要
cDNA libraries were constructed from the leaves of a rice (Oryza sativa L.) salt tolerancevariety Tesan抋i 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blottinganalysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept onincreasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysisindicated that there were no homologies between the three cDNA clones and any known gene. The threecDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8,which was a salt tolerance parent of Tesan抋i 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 werelocated on chromosomes 1, 3 and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near theregions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DHpopulation in a parallel study.
从150 mmol/L盐胁迫3 h和没有胁迫的耐盐水稻(Oryza sativa L.)品种特三矮2号叶片中提取mRNA,构建cDNA文库.通过示差筛选,得到3个盐胁迫应答cDNA克隆Ts1、Ts2和Ts3.Northerd分析表明,3 h的盐胁迫可使Ts1、Ts2和Ts3的转录水平明显上升;3～24 h期间,Ts1和Ts2的转录水平继续上升,而Ts3的转录水平则下降.序列分析表明,这3个cDNA克隆与已知功能的基因没有同源性.利用特三矮2号的耐盐亲本ZYQ8和粳稻JX1 7组合构建了DH群体和分子标记连锁图谱,将Ts1、Ts2和Ts3分别定位在第1、3和7染色体上.值得注意的是,Ts1、Ts2和Ts3与用同一群体定位的主效和微效耐盐QTL位于同一或相邻区域.
基金
国家自然科学基金(39970409)
农业部水稻生物学重点实验室开放项目(0003103)。~~
