摘要
目的 探讨抑癌基因蛋白p16和 p63在各型甲状腺肿瘤中的表达及其意义。 方法 采用免疫组织化学 (EnvisionTM)法检测 60例甲状腺肿瘤细胞中 p16和 p63蛋白的表达情况。 结果 甲状腺腺瘤中 p16及 p63蛋白阳性表达率分别为 60 .0 % ( 18/3 0 )和 46.7% ( 14 / 3 0 ) ,明显高于甲状腺癌 ( 3 3 .3 % ,10 / 3 0 ;2 0 .0 % ,6/ 3 0 ) ,有显著性差异 (P <0 .0 5 )。甲状腺乳头状腺瘤中 p16和p63蛋白阳性表达率分别为 80 .0 % ( 12 / 15 )和 60 .0 % ( 9/ 15 ) ,明显高于甲状腺乳头状腺癌中阳性率 ( 3 3 .3 % ,5 / 15 ;2 0 .0 % ,3 / 15 ) ,有显著性差异 (P <0 .0 5 )。甲状腺滤泡状腺瘤中 p16和p63蛋白阳性表达率分别为 40 .0 % ( 6/ 15 )和 3 3 .3 % ( 5 / 15 ) ,高于甲状腺滤泡状腺癌中其阳性表达率 ( 3 3 .3 % ,5 / 15 ;2 0 .0 % ,3 / 15 ) ,但无显著性差异 (P >0 .0 5 )。结论 检测甲状腺癌中p16和 p63蛋白表达水平 。
Objective To discuss the expression of p16 and p63 protins in thyroid neoplasm and its significance.Methods The expression of p16 and p63 Proteins was examined by immunohistochemical method(Envision TM) in 60 thyroid neoplasms.Results The positive expression rates of p16 and p63 were significantly higher in thyroid adenoma than in thyroid carcinoma (60.0% and 46.7% vs 33.3% and 20.0%,P<0.05).The positive expression rates of p16 and p63 were significantly higher in thyroid papillary adenoma than in thyroid papillary carcinomas(80.0% and 60.0% vs 33.3% and 20.0%,P<0.05).The positive rates of p16 and p63 was significantly higher in thyroid follicular adenoma than in thyroid follicular carcinomas(40.0% and 33.3% vs 33.3% and 20.0%,P>0.05).Conclusion Detection of p16 and p63 protein has a significance for diagnosis and prognosis of thyroid neoplasms.
出处
《实用癌症杂志》
2003年第6期646-648,共3页
The Practical Journal of Cancer
