摘要
目的:研究碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)对培养兔纤维软骨细胞增殖的影响,探讨其作用机制。方法:培养1月龄新西兰兔半月板纤维软骨细胞,以1,10,100μg/L的bFGF作用细胞,以四氮甲基唑蓝(MTT)比色法检测细胞的增殖情况,并在10μg/LbFGF作用下,采用流式细胞技术进行细胞周期亚时相分析。结果:在1～100μg/L范围内bFGF对培养纤维软骨细胞的增殖均有促进作用,10μg/LbFGF即可有显著效果。实验组细胞周期较对照组明显缩短,DNA合成前期(G1期),DNA合成期(S期)和分裂前期及分裂期(G2M)的时间分别为14.58,12.30,11.43h,对照组分别为22.77,17.90,20.62h。结论:bFGF对培养的半月板纤维软骨细胞增殖有促进作用,能缩短纤维软骨细胞DNA合成G1期及G2M期,从而缩短细胞周期、促进细胞分裂增殖。
AIM:To investiga te the effects of basic f ibroblast growth factor(bFGF)on proliferation of cultu red rabbits f ibrochondrocytes and its functional mecha-nism.METHODS:Meniscus fibrochondrocytes from 1-month New Zealand rabbits were cultured and then wer e treated w ith bFGF at the concentration of 1,10and 100μg /L.The prol iferation of fibrochon drocytes was assayed by methyl thiazolyl terazolium(MTT )colorimetric method.Sub-phase analysis of cell cycle was also investigated b y flow c ytometer with the effect of bFGF at 10μg /L.RESULTS:The study sho wed the bFGF at doses ra nging from 1to 100μg /L enhanced the proliferatio n of fibr ochondrocytes,especially in the concen-tration of 10μg /L.Cell cycle in bFGF group was shorter than that in control group.The time of DNA pre synthetic p hase(G 1 ),Synthesis phase(S)and prophase and mitotic phase(G 2 M)in control group were 22.77,17.90an d20.62hours,while th ose in bFGF grou p were 14.58,12.30and 11.43hours respectively.bFGF can enhance the proliferation o f fibrochondrocytes in vitro.T he time for G 1 phase and G 2 M phase can be shortened by bFGF so as to shorten the cell cycle and promote proliferation of cells.
出处
《中国临床康复》
CSCD
2004年第2期238-239,共2页
Chinese Journal of Clinical Rehabilitation
