摘要
通过花粉管通道法用非洲菊基因 gaga1转化N2 899(质核互作雄性不育保持系 )、通州豆、95 - 7等大豆受体。处理的花朵总数为 4 30 0朵 ,共获 30 0 0多粒种子。经卡那霉素田间鉴定 ,获得了 14株抗卡那霉素的幼苗。通过抗性标记基因nptⅡ和目标基因 gaga1的PCR检测 ,发现有两株N2 899具有阳性信号 ,初步证明非洲菊基因 gaga1已整合到这两株大豆的基因组中。形态分析表明 :与未转化N2 899相比 ,转基因大豆株型矮小 ,始花期提前 ,盛花期花数量较少 ,结荚不多。显微分析发现 ,与对照相比转基因大豆花器官结构没有明显改变。
Transformation of soybean with gaga1 was conducted using several accessions includingN2899, Tongzhoudou, 95-7etc. through pollen tube pathway. About 3000 seeds were obtained from 4300 flowers treated with Ti plasmid pHTT613. By indirect identification method with Kanamycin in the field, 14 plants obtained were found resistant to Kanamycin. These 14 plants were examined by PCR with NPTⅡ and gaga1 specific primers. Two N2899 plant showed positive signals. This indicated preliminarily that gaga1 was integrated into the genome of the two plants. These two transgenic plants were shorter and had less flowers during full blooming period, but no obvious modification was found in floral structure compared to untransformed N2899. The detailed characterization of transgenic plants shall be carried out in further generations.
出处
《大豆科学》
CAS
CSCD
北大核心
2003年第2期79-82,共4页
Soybean Science
基金
国家转基因植物研究与产业化专项 (J0 0 -B -0 0 3 -0 7)
教育部霍英东青年教师基金 ( 710 2 4)
江苏省青年科技基金(BQ980 33)
关键词
发育调节基因
遗传转化
花粉管通道
大豆
Regulatory genes
Genetic transformation
Pollen tube pathway
Soybean
