摘要
土壤盐渍是限制杜鹃属(RhododendronL.)植物分布与园林应用的主要因素之一。为探究杜鹃在盐胁迫中的抗性分子机理,以2个杜鹃品种‘江南春早’(R.‘Jiangnan Chunzao’)和‘胭脂密’(R. obtusu‘Yanzhi Mi’)为材料,进行盐胁迫处理,然后采用cDNA-SCoT的方法进行差异表达基因的筛选。结果表明,40个引物能够扩增出90多条带,其长度在100~1 800 bp之间;筛选出32条差异明显的条带进行回收,连接转化,经测序得到了15条高质量的EST序列。其生物信息分析表明,杜鹃盐胁迫下的差异基因主要编码过氧化氢酶、LRR受体样丝氨酸/苏氨酸蛋白激酶At1g63430异构体X1、Shc相关蛋白、光系统Ⅱ蛋白D1以及PTS果糖转运蛋白亚基ⅡABC等功能性蛋白,涉及细胞结构、防御反应、代谢、能量代谢、信号转导、转运、转录等。
Soil salinization is one of the main factors that limit the distribution and landscape application of Rhododendron.To explore the molecular mechanism of Rhododendron resistance to salt stress,R.‘Jiangnan Chunzao’and R.obtusu‘Yanzhi Mi’seedlings were used as the materials to analyse different expressed genes under salt stress by the method of cDNA-SCoT.The results showed that 40 primers could amplify more than 90 bands,most of which were between 100-1800 bp in length.Thirty two bands with obvious differences were selected for recovery.Fifteen high-quality EST sequences were obtained by sequencing after the transformation.Bioinformatics analysis showed that the differential genes in Rhododendron seedlings exposed to salt stress mainly coded catalase,LRR receptor-like serine/threonine protein kinase At1 g63430 Structure X1,Shc-related protein,photosystemⅡprotein D1,PTS fructose transporter subunitⅡABC etc,and involved in cell structure,defense response,metabolism,energy metabolism,signal transduction,transport and transcription etc.
作者
郑福超
耿兴敏
岳远征
苏家乐
贾新平
ZHENG Fuchao;GENG Xingmin;YUE Yuanzheng;SU Jiale;JIA Xinping(College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China;Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2019年第6期1192-1200,共9页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31601785)
江苏高校品牌专业建设工程项目(PPZY2015A063)
