摘要
从全基因组水平鉴定了87个甘蓝NLR基因家族成员,并对其进行了进化分析和表达分析。基因结构和motif组成分析表明,87个基因可分为5个亚类:NB-LRR(34个)、CC-NB-LRR(6个)、TIR-NB-LRR(45个)、RPW8-NB-LRR(1个)和CC-TIR-NB-LRR(1个);染色体定位分析表明,该家族基因在9条染色体上呈不均匀地单一或成簇分布;进化分析表明,甘蓝35个NLR基因与白菜NLR基因存在同源关系,其余52个基因为甘蓝特有;利用转录组数据分析了参与甘蓝枯萎病、黑腐病、根肿病、霜霉病、白粉病等抗性应答相关的差异表达基因,发现参与不同病害胁迫抗性应答反应的NLR基因差异较大,这可能与不同NLR基因对不同病原特异性识别有关。
In this study,based on the reference genome we identified 87 NLR family genes in Brassica oleracea and performed evolution,comparative,and expression analysis.Gene structures and motif composition analysis indicated that the 87 NLR genes were assigned to five subclasses:NB-LRR(34),CC-NB-LRR(6),TIR-NB-LRR(45),RPW8-NB-LRR(1)and CC-TIR-NB-LRR(1).Chromosome location analysis revealed that the NLR genes were unevenly distributed in single or in clusters on nine chromosomes.Evolution analysis showed that 35 NLR genes were homologous with Chinese cabbage,while the other 52 genes were specific to cabbage.Expression profiling of NLR genes identified several differential expression genes related to resistance to Fusarium wilt,black rot,clubroot,downy mildew and powdery mildew in Brassica oleracea,and results showed that expression of NLR genes participating in resistance response to various diseases exhibited significant difference,which may indicate the specific recognition of NLR genes for diverse pathogens.
作者
邢苗苗
刘星
孔枞枞
杨丽梅
庄木
张扬勇
王勇
方智远
吕红豪
XING Miaomiao;LIU Xing;KONG Congcong;YANG Limei;ZHUANG Mu;ZHANG Yangyong;WANG Yong;FANG Zhiyuan;LüHonghao(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Beijing 100081,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2019年第4期723-737,共15页
Acta Horticulturae Sinica
基金
国家重点研发计划项目(2017YFD0101804)
国家自然科学基金项目(31701927)
中央级公益性科研院所基本科研业务费项目(Y2018YJ04)
中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)
国家现代农业产业技术体系建设专项资金项目(nycytx-35-gw01)
关键词
甘蓝
NLR家族基因
抗病
表达分析
Brassica oleracea
NLR family gene
resistance
expression profiling
