摘要
通过蛋白序列同源比对和PCR技术从黄瓜中克隆获得了调控蜡质合成相关基因CER7的序列,命名为CsCER7。系统发育进化树分析发现CsCER7与番茄SlCER7进化距离较近;荧光定量PCR、GUS染色、原位杂交和亚细胞定位结果表明:CsCER7在黄瓜植株中各个器官均有表达,果实中在表皮细胞处特异表达;启动子序列分析显示CsCER7的启动子含有ABA应答的顺式作用元件MYC和ABREs,同时荧光定量PCR的结果也证明了在ABA处理下CsCER7的表达量明显升高,且蜡质总含量也明显增多。据此推测,CsCER7响应ABA,表达量上调,进一步影响果皮蜡质的形成。
CsCER7,a homology exonucleases gene of Arabidopsis thaliana CER7,was recently cloned from cucumber(Cucumis sativus L.). Phylogenetic reconstruction indicated that CsCER7 has closer relation with SlCER7. CsCER7 expression was characterized by RT-PCR,GUS staining,in suit hybrid(ISH). The results showed that CsCER7 is expressed in tested organs,especially in the epidemic cells of fruits. A GFP fusion protein analysis showed that CsCER7 protein is localized in the nucleus and partial cytoplasm where AtCER7 protein is also localized previously reported. Promoter prediction showed that it contains several cis-acting elements such as MYCs and ABREs which response to ABA. RT-PCR analyses that expression of CsCER7 in cucumber fruit peel is increased under treatment with ABA. And wax load of fruit is also improved upon ABA treatment. Collectively,these results indicated that CsCER7 acts response to ABA which induces its higher expression,and further changes the accumulation of fruit cuticular wax.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第4期661-671,共11页
Acta Horticulturae Sinica
基金
国家高技术研究发展计划(‘863’计划)项目(2012AA100103)
国家科技支撑项目(2013BAD20B01)
国家公益性行业(农业)项目(201203003)
北京市科技计划项目(D131100000713001)
北京市产业技术体系创新团队果类蔬菜项目
