摘要
目的建立高效液相色谱法同时测定温经汤中绿原酸、隐绿原酸、甘草酸、桂皮醛、丹皮酚和吴茱萸碱的含量。方法采用Waters Xselect HSS T3色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%甲酸溶液为流动相梯度洗脱,设置流速为0.8 mL·min^(-1),柱温为25℃,进行全波长扫描。结果绿原酸、隐绿原酸、甘草酸铵、桂皮醛、丹皮酚、吴茱萸碱分别在30.02～150.08,30.07～150.36,68.82～344.08,10.10～50.50,10.21～51.06,10.18～50.90μg·mL^(-1)范围内浓度与峰面积呈良好线性关系,相关系数分别为0.999 9,0.999 8,0.999 6,0.999 9,0.999 5,0.999 5。结论该方法准确简便,稳定可靠,可用于温经汤名方开发的质量控制。
Objective To establish a method for the content determination of six water-soluble ingredients(chlorogenic acid, cryptochlorogenic acid, glycyrrhizic acid, cinnamaldehyde, paeonol and evodiamine) in Wenjing decoction by HPLC. Methods The Waters Xselect HSS T3 column(4.6 mm×250 mm, 5 μm) was used, with acetoniteile-0.1% formic acid solution as the mobile phase for gradient elution. The temperature of column was 25 ℃ at a flow rate of 0.8 mL·min-1 with full UV wavelength scan. Results Under the above chromatographic conditions, chlorogenic acid, cryptochlorogenic acid, glycyrrhizic acid ammonium salt, cinnamaldehyde, paeonol and evodiamine showed good linear relationship within the ranges of 30.02-150.08,30.07-150.36,68.82-344.08,10.10-50.50,10.21-51.06 and 10.18-50.90 μg·mL-1, respectively. The correlation coefficient was 0.999 9, 0.999 8, 0.999 6, 0.999 9, 0.999 5 and 0.999 5, respectively. Conclusion This method is accurate, simple, stable and reliable, and it could be taken as an effective method for controlling the quality of Wenjing decoction development.
作者
路娟
柴瑞平
陈曦
LU Juan;CHAI Ruiping;CHEN Xi(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China)
出处
《医药导报》
CAS
北大核心
2019年第4期478-481,共4页
Herald of Medicine
基金
国家自然科学基金面上项目(81673667)
中国医学科学院医学与健康科技创新工程经费资助项目(2016-I2M-3-015
2017-I2M-B&R-09)
云南省中青年学术带头人后备人才项目(2013HB099)
关键词
温经汤
水溶性成分
含量测定
色谱
高效液相
Wenjing decoction
Water-soluble ingredients
Content determination
Chromatography,high performance liquid
