摘要
为提高色素萃取法的灵敏度,用苯乃辛作检样,先从四大类的70种色素中选出新类型酸性色素:二甲苯蓝。再用苯乙托品与苯乃辛两种胺对二甲苯蓝进行了吸收蜂、pH值的选定、检出限、检量线、此蓝与两种胺分子的配合比、本测定法的精密度,五种胺配合物的吸收系数比较等各项研究。并讨论了这一新类型色素的基本分子构造与测定灵敏度提高的原由。
In order to increase the sensitivity of the acid dye determination method, we have tested 70 kinds of acid dyes which possess sulfonic acid or carboxylic acid radical. These dyes, including sulfonphthaleins, azo dyes, triarylmethanes, nitrophenylamines, etc., have not yet been reported in the literature for the determination of amines. It is observed that only the dyes which possess sulfonie acid radical are useful in this method. Among these dyes, 10 kinds were selected for further examination, the result shows that xytene cyanol (XC) is one of two best dyes. The limits of identification of this dye for bencthtropine [1, 1-diphenyl-2 (3-tropanyl) ethene] and benactyzine (benzilic acid 2-diethylamino-ethyl ester) are 17 ng/ml and 36 ng/ml respectively, The extinction coefficients (E_(1cm)^(1%)) of these dye-amine complexes are 4.2～6.2 times as high as those of bromothymol blue (BTB), but for more hydrophilic amines, E_(1cm)^(1%) are lower than those obtained from ETB. Chloroform extract of the complex shows greenish blue color, λ max 628.5 nm. For the determination of amines, the suitable concentration of XC is 4×10^(-3) M. The calibration curve is a straight line in the range of 0～10 μg/ml. With XC concentration of 4 x 10^(-3) M, the mole ratio of dye-amine complex in the chloroform extract for benactyzine is XC: Benactyzine=2: 1, and with concentration of 4×10^(-4) M=1: 1. But, in the case of benethtropine, the mole ratio with different dye concentration(4×10^(-3) M～4×10^(-4) M) remains 2.7: 1 (XC: amine). The coefficient of variance for the determination of 0.2 μg/ml benethtropine or benactyzine is within±5% and ±1～2% for 2.5 μg/ml and 5.0 μg/ml. This dye is a new type acid dye for the determination of amines, and the following portion is considered to be the fundamental structure:
出处
《药学学报》
CAS
1985年第12期923-930,共8页
Acta Pharmaceutica Sinica
