摘要
【目的】探明球孢白僵菌对不同寄主植物来源蚜虫侵染致病力的差异,为烟蚜高毒力杀虫真菌菌株筛选提供依据。【方法】以烟草品种K326和红花大金元为烟蚜来源寄主,采用浸渍法进行生物测定,运用"时间—剂量—死亡率"模型分析侵染致病的时间和剂量效应,利用生殖力表法分析接种处理后烟蚜的生殖力特征。【结果】球孢白僵菌Bb0816菌株对红花大金元和K326烟草品种烟蚜均具有较强的侵染致病力,符合"时间—剂量—死亡率"模型模拟,侵染效应常数分别为0.527和0.532。接种处理后,红花大金元品种烟蚜的死亡率上升最快,在第4～5天达到死亡高峰,而K326烟草品种烟蚜则于第5～6天才达到较高死亡率。从剂量效应来看,该菌株对红花大金元烟草上烟蚜同一时间点的LC_(50)和LC_(90)也小于K326。从时间效应来看,在相同接种剂量下,该菌株对红花大金元烟蚜的LT_(50)和LT_(90)均小于K326;从烟蚜生殖力参数来看,在1.35×10~5～1.35×10~8孢子/mL密度下,红花大金元上烟蚜的内禀增长率rm、净生殖率R_0均低于K326烟蚜,也显著低于对照;在1.35×10~3、1.35×10~4和1.35×10~5孢子/mL处理下,红花大金元和K326烟草上烟蚜的世代历期间无显著差异,而在高浓度的1.35×10~7和1.35×10~8孢子/mL处理下红花大金元烟草上烟蚜世代历期显著小于K326烟蚜。【结论】烟蚜寄主烟草品种差异影响球孢白僵菌对烟蚜的毒力,在一定剂量范围内,球孢白僵菌对红花大金元烟蚜的侵染致病作用强于K326烟草烟蚜。
[Purpose]To verify the pathogenicity of Beauveria bassiana against the Myzus persicae(Sulzer) feeding on the tobacco variety of Honghuadajinyuan(Hongda) and K326 and provide support for the higher virulent fungi isolates screening.[Method]The pathogenicity of B. bassiana Bb0816 on M. persicae was collected from Hongda and K326 by using the immersion method, the time-dosage effect was evaluated using the Time-Dosage-Mortality Model.[ Result] B. bassiana Bb0816 showed a higher virulence against the M. persicae feeding on the tobacco variety of Hongda and K326, it was inconsistent with the Time-Dosage-Mortality Model, the infection efficacy indexes were 0.527 and 0.532 for M. persicae feeding on Hongda and K326, respectively. The mortality increased with the increase of the time after the inoculation, but it increased quickly on the M. persicae feeding on Hongda than that on K326, the maximum mortality was on the 4th to 5th day for M. persicae feeding on Hongda, but on the 5th to 6th day for M. persicae feeding on K326. From the result of the infection dosage, the LC50 and LC90 for M. persicae feeding on Hongda were lower than feeding on K326. From the result of the infection time, the LT50 and LT90 for M. persicae feeding on Hongda was lower than feeding on K326 under the same conidia concentration. From the fecundity factors of M. persicae, the rm, R0 for M. persicae feeding on Hongda was lower than feeding on K326 under the conidia concentration of 1.35×105 conidia/mL to 1.35×108 conidia/mL, and they were all lower than that in CK. No significance difference was found for the generation stage of M. persica feeding on Hongda and K326 under the conidia concentration of 1.35×103, 1.35×104 and 1.35×105 conidia/mL,but it was lower on M. persica feeding on Hongda than on K326 under the concentration of 1.35×107 and 1.35×108 conidia/mL.[Conclusion]The variety of tobacco affects the pathogenicity of B. bassiana against the M. persica, the virulence was higher on the M. persica feeding on Hongda and K326 under the
作者
徐继伟
李丽
肖志新
李正跃
陈斌
XU Jiwei;LI Li;XIAO Zhixin;LI Zhengyue;CHEN Bin(State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,College of Plant Protection,Yunnan Agricultural University,Kunming,650201,China;Baoshan Branch of Yunnan Provincial Tobacco Company,Baoshan 678000,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2019年第2期216-222,共7页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省烟草公司科技计划项目(2015YN24)
云南农业大学研究生创新项目(2016ykc13)
