摘要
目的 研究上海地区汉族人群中单胺氧化酶B(MAOB)基因第二内含子中鸟嘌呤、胸腺嘧啶 (GT)二碱基重复的微卫星多态位点与帕金森病 (Parkinson’sdisease ,PD)易患性之间的关系。方法 采用扩增片段长度多态法 (Amp FLP) ,在上海汉族人群中选择 6 7例散发PD患者为PD组和2 0 4名健康者为对照组 ,运用微卫星荧光标记 半自动基因分型技术精确计算出微卫星等位基因片段大小 ,进而分析该多态在PD易患性中的作用。结果 该位点短片段 (≤ 170bp)等位基因的分布在PD组中明显高于与对照组 (χ2 =11 2 8,P =0 0 0 1) ,而 172bp等位基因在PD组中亦明显低于与对照组 (χ2 =5 16 ,P =0 0 2 3)。结论 MAOB基因GT二碱基重复多态位点与本组PD患者的易患性有关。
Objective To investigate the relationship of the GT dinucleotide repeat polymorphism in intron 2 of the monoamine oxidaseB gene (MAOB,Xp15.21-4) and Parkinson’s disease(PD).Methods The samples are recruited from Shanghai Han population,including 67 sporadic PD patients and 204 health controls.Using Amp-FLP and microsatellite fluorescence-based semiautomatic genotype analysis technique,the DNA fragment length of microsatellite polymorphism was precisely calculated.Furthermore,the role of the microsatellite in sensitivity to PD was observed. Results With χ 2 test,the (GT)n repeat alleles being shorter than or equal to 170 base pairs in the intron 2 of the MAOB gene were found significantly associated with PD(χ 2=11.28, P =0.001).The distribution of 172 base pair allele in PD cases was significantly lower than those controls(χ 2=5.16, P =0.023).Conclusion (GT)n repeat polymorphism in MAOB gene is probably associated with PD’s onset.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2003年第6期418-421,共4页
Chinese Journal of Neurology
