摘要
通过研究不同年龄和不同性别绒山羊背最长肌之间的差异表达基因,筛选影响绒山羊肉品质的候选基因。本研究基于RNA-Seq技术对4个绒山羊背最长肌的转录组进行高通量测序,在测序评估的基础上,对差异表达基因进行筛选、功能注释和富集分析。本研究通过CLC Genomics Workbench 6.0软件共找到263个候选基因,其中包含123个高表达有利基因和140个高表达有害基因。GO功能注释结果显示,高表达有利基因主要与骨骼肌的生长发育、细胞器的形成和蛋白结合功能有关;高表达有害基因主要与脂质代谢、细胞骨架以及结合功能有关。利用KEGG数据库作为参考,这些基因主要参与糖酵解/糖异生、丝裂原活化蛋白激酶、凝血-补体级联反应和色氨酸代谢等通路中。结合其他家畜基因组学研究,ADIPOQ、PDK4和CD36可能作为参与肉品质重要的候选基因。该结果为绒山羊肉品质的改善以及候选基因的研究提供了理论依据。
To screen candidate genes which effect on meat quality of cashmere goats,we explored differentially expressed genes of longissimus dorsi in cashmere goats with different ages and genders.The high-throughput sequencing for transcriptome of longissimus dorsi in cashmere goats was performed by RNA-Seq.Differentially expressed genes were selected as candidates genes and enriched based on GO and KEGG database after quality control of RNA-Seq.The 263 candidate genes including 123 beneficial and 140 deleterious genes were selected by CLC Genomics Workbench 6.0software.The results of GO enrichment showed that the highly expressed beneficial genes were primarily related to growth and development of skeletal muscle,organelle formation and protein binding.The highly expressed deleterious genes were primarily related to lipid metabolic process,cytoskeleton and binding.According to the KEGG database,these genes were mainly involved in glycolysis/gluconeogenesis' mitogen-activated protein kinase,complement and coagulation cascades and tryptophan metabolism.As example,ADIPOQ,PDK4 and CD36mightbe important candidate genes involved in meat quality,when combining with other livestock genomics study.These results provided theoretical basis for improving meat quality and studying candidate genes in cashmere goats.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2015年第8期1300-1307,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30960246)
内蒙古自然科学基金(2010Zd11)
"内蒙古自治区草原英才"与"内蒙古自治区高等学校创新团队发展计划"(NMGIRT1106)
国家绒毛用羊现代农业产业技术体系(CARS-40-05)
