摘要
本研究旨在探讨3种冷冻方法(CLV法、OPS法和Straw法)对猪MII期卵母细胞玻璃化冷冻后微丝、脂肪颗粒分布及其超微结构的影响。结果显示:(1)CLV(Cryoloop vitrification)法冷冻速率最高,达42 639℃·min-1;(2)CLV法微丝正常分布率(47.06%)最高,显著高于OPS(Open pulled straw)法的38.04%和Straw法的30.95%(P<0.05)。(3)CLV法和OPS法的脂肪颗粒正常分布率之间没有显著差异(P>0.05),但两者均显著高于Straw法(P<0.05);(4)解冻后卵母细胞超微结构观察结果显示,大的脂肪颗粒含量明显减少;大部分脂肪颗粒冷冻后呈均质化状态,只有很少的异质化脂肪颗粒存在。试验结果表明,冷冻过程中卵母细胞脂肪颗粒破坏严重,增加冷冻速率能降低对微丝的损伤,从而减少对脂肪颗粒异常分布的影响。
The purpose of this study was to determine the microfilament distribution,lipid droplets and their ultrastructure changes that resulted from vitrified porcine MII-stage oocytes with 3vitrification techniques(Straw method,CLV method and OPS method).The results showed that:(1)The cooling speed of CLV method was the highest,and got 42 639℃·min-1.(2)CLV method got the highest normal distribution rate(47.06%),and it was much higher than OPS method(38.04%)and Straw method(30.95%).(3)There was no significant differences in the normal distribution rate of lipid droplets between CLV and OPS methods after thaw,but they were much higher than that of Straw method(P<0.05).(4)Decreased content of large lipid droplets in thawed oocytes were found out after ultrastructural observation with transmission electron microscopy(TEM),and most of lipid droplets in thawed oocytes were homogeneous and very few of them were heterogeneous.These results concluded that the lipid droplets and their distribution af-ter vitrification were damaged greatly.Improving cooling speed could decrease the injury of microfilament,and then reduce the damage degree of distribution in lipid droplets.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第7期1097-1103,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
上海市科委农业成果转化专项(123919N0700
133919N1700)
国家自然科学基金(31372315)
高繁殖力转基因猪新品种培育(2013ZX08006-005)
