摘要
目的:通过对慢性阻塞性肺疾病(Chronic Obstructive Pulmonary Disease,COPD)患者与正常人血清谷胱甘肽(glutathione,GSH)和白介素18(IL-18)的表达检测,探讨其在COPD中的作用。方法:采集32例COPD患者、30例正常人血清,测第一秒用力呼气容积/用力肺活量(FEV1.0/FVC%)提示肺功能,通过比色法和酶联免疫吸附法测定不同阶段血清中GSH和IL-18水平。结果:COPD稳定期较急性期肺功能显著提高(P<0.05),GSH水平明显升高(P<0.05),IL-18水平显著降低(P<0.05);正常组较COPD组有显著差异(P<0.05)。结论:随着COPD患者的转归,GSH血清水平增高而IL-18水平降低,提示GSH可能是COPD的保护性因素,而高水平的IL-18与COPD发病相关。
Objective:To discuss the function of difference expression of GSH (glutathione, GSH) and IL-18 in patients of COPD. Methods: Collect the serum of 32 COPD patients and 30 normal people. According the FEV1.0 (forced expiratory volume in one second,FEV1.0) and FEV1.0/FVC (forced vital capacity rate of one second) to detect the lung function of COPD patients. The colorimetric method and the enzyme-linked immunosorbent assay (ELISA) method were used to detect the level of GSH and IL-18 in serum of the different stage of COPD and normal person. Then we analyzed the difference in different groups. Results: Acute stage COPD patients were transfered to stable stage after drug treatment. Lung function indices (FEV1.0 and FEV1.0/FVC) and GSH level improved apparently in stable stage patients (p<0.05) while the level of IL-18 was significantly reduced in stable stage (p<0.05). These indexes have significantly difference in COPD patients and normal control. GSH level in normal control people was also high while IL-18 was low. GSH and IL-18 in different group have no relevance (p>0.05). Conclusion:With the improvement of the symptoms of COPD patients, GSH level in serum was increased while IL-18 was decreased. That means GSH is a protective factor for COPD patients, while high level IL-18 was related with morbidity of COPD.
出处
《新疆医学》
2014年第4期12-16,共5页
Xinjiang Medical Journal
