摘要
目的建立翼核果中的翼核果素及翼核果醌-Ⅰ的含量测定方法,对翼核果素及翼核果醌-Ⅰ抗氧化活性进行初步评价。方法采用HPLC对翼核果药材中的翼核果素及翼核果醌-Ⅰ的含量进行测定,并进行方法学考察;通过测定翼核果素及翼核果醌-Ⅰ对DPPH自由基的清除能力,以半数清除浓度(IC50)值作为评价清除DPPH自由基能力的指标。结果翼核果药材中翼核果素及翼核果醌-Ⅰ的含量分别为0.56%,0.10%。2种成分清除DPPH自由基的IC50值分别为1.13,1.63 mg·mL^(-1)。结论 2种成分的定量分析方法分离度良好,重复性好,抗氧化方法简便、快捷,为翼核果的深入研究提供了实验依据。
OBJECTIVE To establish a method for simultaneous determination of ventilagolin and ventiloquinone-Ⅰfrom Ventilago leiocarpa Benth and to evaluate DPPH free radical scavenging for the anti-oxidant activity.METHODS HPLC method was used to determine the content of ventilagolin and ventiloquinone-Ⅰand methodologically inspected.Taking IC50 as the evaluating criterion of scavenging capability by determining the DPPH free radical scavenging rate of ventilagolin and ventiloquinone-Ⅰ.RESULTS The contents of ventilagolin and ventiloquinone-Ⅰwere 0.56%and 0.10%.The DPPH free radical scavenging capability of IC50 were 1.13,1.63 mg·mL-1.CONCLUSION The method has good separation and good reproducibility.The method is convenient and rapid,as well as provide an experimental to intense research of Ventilago leiocarpa Benth.
作者
胡筱希
陆国寿
黄周锋
黄建猷
谭晓
甘吉丽
HU Xiaoxi;LU Guoshou;HUANG Zhoufeng;HUANG Jianyou;TAN Xiao;GAN Jili(of Traditional Medical and Pharmaceutical Sciences,Nanning530022,China;Guangxi Key Laboratory of Traditional ChineseMedicine Quality Stardands,Nanning530022,China;Guangxi University of Chinese Medicine,College of Pharmacy,Nanning530022,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2019年第7期820-823,共4页
Chinese Journal of Modern Applied Pharmacy
基金
广西自然科学基金项目(2016GXNSFAA380092)
广西中药质量标准研究重点实验室自主研究课题(桂中重自201506
桂中重自201601)
