摘要
目的开发使用普通扫描仪/数码像机等就能作平面色谱分析、指纹谱图相似度计算、高密度点阵检测的多功能软件。方法根据数字化图像处理技术原理,用Visual C++语言和Delphi语言编程。以改进的特征曲线重合率公式计算相似度。结果能给出平面色谱定性的Rf值;能将平面色谱的可视及荧光斑点定量数值化;能计算指纹图谱相似度;能清晰分辨6400点/cm^2的可见光点阵,及以1μg/mL核黄素溶液点构的64点/cm^2的荧光点阵(每点1ng核黄素);待测图像文件格式可为BMP等通用格式;数据自动列表,可编辑,可以Excel格式保存。结论系统性能良好,整体优化水平高,人机交换界面友好,操作简便,设备简单、价格低廉。
OBJECTIVE To develop a multiple-function soft for Planar Chromatography analysis,calculation of similarity of Chinese traditional medicines chromatographic fingerprint,detection of high density array,by using a cheap common scanner or digital camera/ultraviolet analyzer.METHOD Program basing on the principle of computer-associated digital image processing by using VC+ + and Delphi language,calculate similarity using the modified coincident rate formula for characteristic curves.RESULTS 1) Rf value can be obtained;a visible and fluorescence spot can be transferred quantitatively into gray scale value;similarity of Chinese traditional medicines fingerprint can be calculated;an visible array of 6400 dots/cm ' and a fluorescent array of 64 dots/cm' constructed with a lactochrome solution of lμg/mL,about lng lactochrome/dot,can be identified clearly.Formulation of a file can be Use BMP;Data can be listed automatically,and can be edited,and saved as an Excel file。 CONCLUSION The system has excellent characteristics and optimized well as a whole,with a friendly human-machine exchange interface,simplicity of operation,cheaper apparatus.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2004年第S1期6-9,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
数字图像处理
平面色谱
点阵
指纹图谱
相似度
digital image processing
planar chromatography
array
fingerprint similarity
