摘要
目的:探讨去甲斑蟊素对神经胶质瘤U87MG细胞生物学行为的影响。方法:用MTT法检测不同浓度的去甲斑蟊素对U87MG细胞增殖的抑制作用。用ELISA实验检测血管内皮生长因子(VEGF)蛋白的表达水平。结果:MTT结果表明去甲斑蟊素对U87MG细胞的增殖有抑制作用,并随浓度增加抑制作用增强;ELISA实验提示去甲斑蟊素抑制U87MG细胞分泌VEGF蛋白。结论:去甲斑蟊素可以抑制U87MG细胞增殖,抑制肿瘤新生血管的形成。
Objective:To explore the effect of norcantharidin on neuroglioma U87MG cell biological behaviour.Methods:The inhibition effects of different concentrations of norcantharidin on U87MG cell proliferation were detected by MTT method.The expression level of vascular endothelial growth factor(VEGF) protein was detected by ELISA test.Results:MTT results showed that norcantharidin had inhibition effect on U87MG cell proliferation,and the inhibition effect increased with the increasing concentration.ELISA test showed that norcantharidin inhibited U87MG cell secretory VEGF protein.Conclusion:Norcantharidin can inhibit U87MG cell proliferation,inhibit the formation of tumor angiogenesis.
出处
《中国社区医师》
2015年第22期5-5,10,共2页
Chinese Community Doctors
