摘要
运用细菌通用16S rRNA特异的引物对,将生防菌BC2000、BC2001和对照菌株用6种内切酶进行PCR—RFLP扩增分析。结果表明,内切酶Hin6I、CfoI的酶切可把7种菌分成4组:A组为BC2000、BC2001;B组为圆褐固氮菌;C组为荧光假单胞菌;D组为拮抗菌LB2、LB3、Apb。内切酶Hin6I、CfoI的酶切图谱谱带清晰,差别明显,可作为生防菌BC2000、BC2001的分子标记图谱。
Several biocontrolling strains were analyzed by using PCR-RFLP with 16S rRNA universal primers of bacteria, which indicated that seven isolations could be separated into 4 groups (Group A: BC2000, BC2001; Group B: Azotobacter chroococcum; Group C: Pseudomonas fluorescens; Group D: LB2, LB3 and Apb) by restriction enzyme Hin6I and CfoI.The binding types of Hin6I and CfoI were clear enough to be molecular labels of BC2000 and BC2001.
出处
《福建农业学报》
CAS
2003年第4期264-267,共4页
Fujian Journal of Agricultural Sciences
基金
福建省中国-白俄罗斯合作项目(2001 I 012)