摘要
该文旨在探索丹参注射液对胎鼠大脑皮质神经干细胞(neural stem cells, NSCs)体外增殖和分化的影响。以丹参注射液丹参生药(1.5 mg/mL)作为计算药物浓度;体外培养NSCs,将其分为正常对照组和丹参注射液处理组,用Cell Counting Kit-8(CCK-8)试剂、Bromodeoxyuridine(BrdU)试剂、共聚焦高内涵分析系统和RT-PCR技术分别检测丹参注射液对NSCs增殖与分化的影响。CCK-8试剂检测结果显示,经1.17μg/mL丹参注射液处理的NSCs细胞,其增殖能力[光度值为(0.408±0.006)]较对照组[光度值为(0.216±0.003)]显著升高(P<0.01);BrdU检测结果显示,经1.17μg/mL丹参注射液处理的NSCs细胞增殖能力较对照组可提高40.83%(P<0.01);共聚焦高内涵分析结果显示,经0.59μg/mL丹参注射液处理的NSCs向神经元方向分化效率较对照组可提高28.73%(P<0.01);RT-PCR结果表明,经丹参注射液处理的NSCs分化7天后,神经元相关基因NeuroD 1、Tuj1等表达量显著上升。结果显示,丹参注射液不仅可提高NSCs的增殖能力,同时可促进其向神经元方向的分化。
Purpose of this study is to investigate the effects of Salvia miltiorrhiza injection on the proliferation and differentiation of neural stem cells(NSCs)derived from cerebral cortex of fetal mouse.The drug concentration was calculated from the concentration of crude drug of Salvia miltiorrhiza injection(1.5 mg/mL).The cortex neural stem cells were cultured in vitro and divided into control group and Salvia miltiorrhiza injection treatment group.The drug effects on the proliferation and differentiation of NSCs were detected by cell proliferation assay CCK-8,Brdu,confocal high-content analysis system and RT-PCR.Results of CCK-8 and BrdU detection showed that treating with 1.17μg/mL of Salvia miltiorrhiza injection,the NSCs’capacity of proliferation can be significantly improved than that of the control group(P<0.01);Confocal high-content analysis result indicated that the Salvia miltiorrhiza injection can apparently promote the differentiation of NSCs into neurons(P<0.01);RT-PCR results also demonstrated that the neuron-specific gene expression,such as NeuroD1 and Tuj1,increased significantly when NPC cells were pre-treated with Salvia miltiorrhiza injection.From the results,it can be concluded that Salvia miltiorrhiza injection can promote the proliferation of neural stem cells and also enhance their capacity of differentiation into neurons.
作者
吴利
汪俊帮
罗玉萍
Wu Li;Wang Junbang;Luo Yuping(School of Life Sciences,Nanchang University,Nanchang330031,China;Shanghai Tongji Hospital,Shanghai200065,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2019年第7期1330-1337,共8页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31660324)资助的课题~~
关键词
丹参注射液
神经干细胞
细胞增殖
神经元方向分化
Salvia miltiorrhiza injection
neural stem cells
proliferation
differentiation into neurons
