摘要
为了制备丙型肝炎病毒分片段抗体检测蛋白质芯片 ,并对其临床应用价值进行评价 ,将基因工程表达的丙型肝炎病毒分片段抗原 ,点至经特殊处理的玻片上 ,制成蛋白质芯片 .收集来自三家临床单位用于临床验证的 90 5份血清标本 .分别用丙肝病毒分片段抗体检测蛋白质芯片、ELISA丙肝病毒抗体检测试剂进行检测 .部分样本同时采用进口RIBA抗体检测试剂进行了检测 ,分别比较蛋白质芯片法与ELISA法以及RIBA试剂的符合率 .结果表明 :a 90 5份血清标本 ,ELISA法检出阳性 2 94份 ,阴性 611份 .阳性标本用蛋白质芯片法检测 ,融合抗原 2 92份显示阳性结果、 2份阴性结果 ,根据蛋白质芯片的核心抗原 ,以及NS3 ,NS4,NS5分片段抗原综合判断确定阳性样本 2 88份阳性 ,阴性样本 2份 ,4份样本结果不确定 .ELISA法检出的 611份阴性标本用两种蛋白质芯片法检测 ,检出阴性均为 611份 .两种蛋白质芯片法与ELISA法的阳性符合率分别为 99 3 %和98 9% ,与ELISA法的阴性符合率均为 10 0 % .用RIBA试剂检测 6份ELISA法为阳性 ,蛋白质芯片法为非阳性的样本 ,结果均为非阳性 .b 2 90份经RIBA试剂确认的阳性标本 10 4份 ,单片段阳性标本 66份 ,阴性标本12 0份 ,用蛋白质芯片法检测 ,检出阳性标本 10 3份 ,单片段阳性标本 61份 ,阴性标本
To prepare and evaluate the clinical application of protein-chip for different HCV antibody detection, 905 serum samples were collected from three different hospitals. The samples were detected by ELISA method and protein-chip method respectively. Inconsistent samples were proved by imported HCV RIBA diagnostic kit. The results showed that ( 1) In all 905 serum samples, 294 positive samples and 611 negative samples were detected by ELISA method. Among the 294 positive samples, 292 positive samples and 2 negative samples were detected by chimeric antigen on the protein-chip and 288 positive samples, 2 negative samples, 4 indefinite samples were detected by the 4 segments of HCV antigens on the protein-chip. Among the 611 negative samples, 611 samples show negative result detected by chimeric antigen on the protein-chip and the 4 segments of HCV antigens on the protein-chip. The coordinate ratio of positive results are 99.3% and 98.9% respectively compared with ELISA method. The coordinate ratio of negative result are the same 100%. The 6 samples that were positive detemined by ELISA method and non-positive by protein-chip method were detected with RIBA reagent. All the 6 samples show non-positive result. (2) 290 samples were detected with RIBA reagent. Among the 290 samples, 104 samples showed positive result, 66 samples showed positive result to single antigen segment, and 120 samples showed negative result. The 290 samples were also detected by protein-chip method. Among the 290 samples, 103 samples showed positive result, 61 samples showed positive result to single antigen segment, and 126 samples showed negative result. The result shows high coordinate ratio between the two methods (P > 0.05). It can be concluded that protein-chip reagent for detection of different HCV antibodies has higher sensitivity and specific than ELISA method and has the same accuracy as imported RIBA reagent. It will be a convenient and low costs reagent for diagnosis of HCV.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2003年第6期935-939,共5页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金重点资助项目( 398890 0 1 )
军队杰出人才基金项目
国家高技术"86 3"计划资助项目 ( 2 0 02AA2Z2 0 32 )~~
