摘要
乳糖不仅可以诱导棉铃虫颗粒体病毒增效蛋白表达,而且表达量与IPTG(异丙基-β-D-硫代半乳糖苷)作为诱导剂时相比,并不低于后者,这样大规模生产棉龄虫颗粒体病毒增效蛋白的成本就可能被降低.乳糖的最适浓度经优化为2.2%～2.5%(W/V).对乳糖的不同除菌处理表明:0.70×105Pa 15min蒸汽灭菌的乳糖可以诱导此增效蛋白表达,这种处理比滤膜过滤除菌更为方便和经济,因此就为棉铃虫颗粒体病毒增效蛋白的生产提供了经济实用的前景.
Lactose was shown to no less competent than Isopropyl-β-D-thiogalactoside (IPTG) in inducing the expression of the ENHANCIN coding gene from Helicoverpa armigera granulosis virus in Eswcherichia coli BL21 (DE3) regulated by a T7 promoter, since the lactose induction could lead to an ENHANCIN band no smaller than the one in IPTG induction on the SDS-PAGE gel. This would decrease the cost of the large-scale ENHANCIN production. The lactose concentration was optimized at 2.2% - 2.5% (w/v) . Different treatments on the lactose sterilization showed that lactose steam- sterilized in 116. 5℃ for 15min could lead to the ENHANCIN production. The convenience and the relatively low cost in its' operation could further decrease the cost of the ENHANCIN production.
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第6期5-9,共5页
Microbiology China
基金
国家发展计划委员会高技术产业化推进项目(No.1999-1699)
