摘要
目的:研究核转录因子-κB(nuclearfactor-κB,NF-κB)在急性肺损伤的动态改变,以期找到NF-κB在肺损伤中的作用。方法:25只雄性小鼠随机分为5组,正常对照及不同时间点致伤组,致伤组腹腔注射脂多糖形成急性肺损伤模型,采用免疫印迹和免疫组化法检测正常鼠及致伤鼠肺组织中NF-κB的表达。结果:脂多糖注射后1hNF-κB即开始增高,3h达高峰,随后开始下降。结论:NF-κB可能是急性肺损伤发病中的一个关键性因子。
Objective:To investigate the function of nuclear factor-êB(N F- êB) in acute lung injury(ALI),the dynamic change of NF-êB in lung of ALI was detected. Methods:Twenty five male mice were divided into five groups at random . There were normal control group and four acute lung injury groups at different time point.The lipopolysaccharide(LPS)was admistrated by intraperitoneal inje ction. Expression of NF-êB was detected by the methods of Western-blot and im munohistochemistry. Results:Expression of NF-êB began to increase after injec ting LPS in the first hour,arrived the peak in the third hour and then decreased . Conclusion:NF-êB may be a key medium in ALI.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第1期49-51,共3页
Journal of Nanjing Medical University(Natural Sciences)
