摘要
目的 :探讨自身免疫反应在丙型肝炎中的作用。方法:应用间接免疫荧光法,以生物薄片马赛克技术制备的冰冻组织细胞切片为抗原与待测血清结合,加入荧光素标记物抗人免疫球蛋白,检测94例丙型肝炎(丙肝)患者血清中抗核抗体(ANA)、抗线粒体抗体(AMA)、抗平滑肌抗体(SMA)、抗肝膜抗体(LMA)和抗肝特异性脂蛋白抗体(LSP)等自身抗体,并根据荧光反映模式判定结果。结果:(1)急性丙肝组患者14例中有6例出现自身抗体阳性,检出率为42.8 %;慢性丙肝组80例中自身抗体阳性者30例,检出率37.5%。两组比较差别无统计学意义。(2)抗 -HCV阳性的64例中自身抗体阳性者28例,检出率43.75 %;HCVRNA阳性的30例中8例抗体阳性,检出率26.67 %。结论:HCV感染过程中机体可出现自身免疫反应。
Objective:To investigate the role of autoimmune reaction in hepatitis C.Methods:ANA,AMA,SMA,LMA and LSP were detected using indirect immunofluorescence technology in 94 patients with hepatitis C.Results:(1) The positive rate of autoantibodies was 42.8%in patients with acute hepatitis C and 37.5%in chronic patients.(2)The positive rate was 43.75%in patients with HCV antibody positive and 26.67%in patients with HCV RNA positive.There was no significant difference between the above two groups.Conclusion:Autoimmune reaction may be present in patients with HCV.Autoantibodies can serve as an important index applied in the diagnosis and treatment of HCV patients.
出处
《天津医药》
CAS
北大核心
2003年第12期770-771,共2页
Tianjin Medical Journal
