摘要
目的 了解多形核中性粒细胞 (PMN)对大环内酯抗生素内在化浓聚的规律及其继发意义。方法 采用体外PMN与红霉素共孵育的方法 ,检测共孵育过程及剌激活化后细胞内外红霉素浓度比的时相动力学改变 ;采用免疫荧光技术检测PMN对红霉素浓聚后细胞膜表面炎性表征膜结合弹力酶的基础水平以及经刺激活化后的改变 ;检测下呼吸道感染大鼠循环注入红霉素浓聚的PMN后 ,支气管肺胞灌洗液中红霉素浓度与正常大鼠的差异。结果 PMN可迅速浓聚红霉素 ,6 0min细胞内 /外浓度比达高峰 16 / 1,而此时膜表面弹力酶水平则明显下降 ;剌激活化浓聚红霉素的PMN ,可导致细胞内 /外浓度比下降 ,而此时膜表面弹力酶表达上升 ,但上升幅度低于正常PMN ;接受浓聚红霉素PMN注射的下呼吸道感染大鼠 ,其气道中红霉素浓度明显高于正常大鼠。结论 红霉素可在PMN中迅速浓聚并导致PMN基础活化水平的降低以及刺激活化反应的抑制 ,刺激活化可导致浓聚红霉素的部分释放 ,此特性有助于浓聚于PMN的红霉素向感染部位的定向释放。
OBJECTIVE To investigate the role and secondary significance of macrolides concentrated within polymorphonuclear neutrophils(PMNs). METHODS PMNs were incubated in vitro with erythromycin. The dynamic changes in the intracellular/extracellular erythromycin concentration ratio(C/E) were detected during the incubating period and after PMNs were activated, meanwhile the PMNs membrane-bound leukocyte elastase(HLE) that marks the inflammatory response of PMNs was measured by immunofluorescence technique. The difference of erythromycin concentration in the bronchoalveolar lavage fluid(BALF) between normal rats and rats with lower respiratory tract infection was detected after the experimental rats were injected intravenously with the PMNs in which erythromycin was concentrated. RESULTS The erythromycin C/E achieved the peak(16/1) within 60 minutes, meanwhile the PMN membrane activated by stimulation, its C/E decreased while the membrane-bound HLE increased, but the extent of this increase was lower than that of normal PMNs. The erythromycin concentration of BALF which collected from the rats with lower airway infection was higher than that from normal rats. CONCLUSIONS Erythromycin could be accumulated within PMNs quickly. The accumulation could induce the decrease in PMNs basic activity and the inhibition of stimulative activity. The partial release of intracellular erythromycin could be induced by stimulating the PMNs. This characteristics is helpful to the release of the erythromycin accumulated within PMNs towards the direction of infective site.
出处
《中华医院感染学杂志》
CAS
CSCD
2003年第12期1101-1103,共3页
Chinese Journal of Nosocomiology
