摘要
AIM: To investigate whether TL-10-transduced dendritic cells (DCs) could induce tolerogenicity and prolong allograft survival in rat intestinal transplantation.METHODS: Spleen-derived DCs were prepared and genetically modified by hTL-10 gene. The level of IL-10 expression was quantitated by ELTSA. DC function was assessed by MTT in mixed leukocyte reaction. Allogeneic T-cell apoptosis was examined by flow cytometric analysis. Seven days before heterotopic intestinal transplantation, 2x106 donor-derived IL-10-DC were injected intravenously, then transplantation was performed between SD donor and Wistar recipient.RESULTS: Compared with untransduced DC, IL-10-DC could suppress allogeneic mixed leukocyte reaction (MLR). The inhibitory effect was the most striking with the stimulator/effector (S/F) ratio of 1:10. The inhibition rate was 33.25 %,41.19 % (P<0.01) and 22.92 % with the S/E ratio of 1:1,1:10 and 1:50 respectively. At 48 hours and 72 hours by flow cytometry counting, apoptotic T cells responded to IL-10-DC in MLR were 13.8 % and 30.1%, while untransduced group did not undergo significant apoptosis (P<0.05). IL-10-DC pretreated recipients had a moderate survival prolongation with a mean allograft survival of 19.8 days (P<0.01),compared with 7.3±2.4 days in control group and 8.3±2.9days in untransduced DC group. Rejection occurred in the control group within three days. The difference between untreated DC group and control group was not significant.CONCLUSION: IL-10-DC can induce allogenic T-cell hyporesponsiveness in vitro and apoptosis may be involved in it. IL-10-DC pretreatment can prolong intestinal allograft survival in the recipient.
AIM:To investigate whether IL-10-transduced dendritic cells (DCs) could induce tolerogenicity and prolong allograft survival in rat intestinal transplantation. METHODS:Spleen-derived DCs were prepared and genetically modified by hIL-10 gene.The level of IL-10 expression was quantitated by ELISA.DC function was assessed by MTT in mixed leukocyte reaction.Allogeneic T-cell apoptosis was examined by flow cytometric analysis.Seven days before heterotopic intestinal transplantation,2×10~6 donor-derived IL-10-DC were injected intravenously,then transplantation was performed between SD donor and Wistar recipient. RESULTS:Compared with untransduced DC,IL-10-DC could suppress allogeneic mixed leukocyte reaction (MLR).The inhibitory effect was the most striking with the stimulator/ effector (S/E) ratio of 1:10.The inhibition rate was 33.25 %, 41.19 % (P<0.01) and 22.92 % with the S/E ratio of 1:1, 1:10 and 1:50 respectively.At 48 hours and 72 hours by flow cytometry counting,apoptotic T cells responded to IL- 10-DC in MLR were 13.8 % and 30.1%,while untransduced group did not undergo significant apoptosis (P<0.05).IL-10- DC pretreated recipients had a moderate survival prolongation with a mean allograft survival of 19.8 days (P<0.01), compared with 7.3±2.4 days in control group and 8.3±2.9 days in untransduced DC group.Rejection occurred in the control group within three days.The difference between untreated DC group and control group was not significant. CONCLUSION:IL-10-DC can induce allogenic T-cell hyporesponsiveness in vitro and apoptosis may be involved in it.IL-10-DC pretreatment can prolong intestinal allograft survival in the recipient.
