摘要
目的 观察单独使用醋酸甲孕酮 (MPA)、十一酸睾酮 (TU)和联合应用对雄性大鼠骨髓和生精细胞微核发生率的影响。 方法 2 4只大鼠分组后 ,分别给予每月一次肌注MPA 75mg/kg、TU 2 5mg/kg及MPA 75mg/kg +TU 2 5mg/kg ;另以等体积植物油溶剂为对照 ,共 3个月。测定各组大鼠给药后骨髓与生精细胞微核率、精子计数与形态和生殖激素变化情况。 结果 与对照组相比 ,实验组大鼠促性腺激素和生精功能均明显抑制 (P <0 .0 5) ;骨髓嗜多染红细胞的微核率无明显升高 (P >0 .0 5) ;生精细胞微核率明显增加 (P <0 .0 5) ;附睾精子不仅数量下降 ,而且畸形率升高 (P <0 .0 5)。 结论 MPA或TU单独或联合使用对体细胞均无致突变性 ,而使生精细胞微核增加 。
Objective: To identify the effect of medroxyprogesterone acetate(MPA), testosterone undecanoate (TU), and MPA+TU on the micronucleus rate in bone marrow cells and sperm cells of male rats. Methods: Twety-four rats had been classified into 4 groups. Each group received injection of plant oil(group C) or TU(25 mg/kg, group T) or MPA (75 mg/kg, group P) or MPA (75 mg/kg) +TU (25 mg/kg) (group PT) monthly for 3 months. Serum sexual hormones (FSH, LH, T), sperm counting, sperm morphology, and the micronucleus rate in bone marrow cells and sperm cells had been analysed. Results: Spermatogenesis of rats undergoing administration of MPA, TU, and MPA+TU had been suppressed. Testes of rats in group P, T and PT atrophied and sperm counting decreased remarkably when compared with group C(P<0.05). There was no statistical difference of the micronucleus rate in bone marrow cells between treatment group and control group(P>0.05). The micronucleus rate in sperm cells in group P, T and PT increased significantly(P<0.05). Conclusions: MPA, TU, and MPA+TU has no significant effect on increasing the incidence of the micronucleus rate in bone marrow cells, whereas it can raise the micronucleus rate in sperm cells. These data show that MPA/TU has no influence on genetic mutation in somatic cells. The augment of micronucleus rate in sperm cells may be associated with the suppression of spermatogenesis.
出处
《生殖医学杂志》
CAS
2003年第6期334-338,共5页
Journal of Reproductive Medicine
