摘要
目的:观察口腔鳞状细胞癌瘤芽(tumorbudding)与上皮–间质转化(EMT)相关性,进一步探讨其与临床病理特征及预后的关系。方法:收集我院2007年至2013年收治的30例口腔鳞状细胞癌患者手术切除的标本,根据HE染色瘤芽数量将其分为2组,低瘤芽组(≤5),高瘤芽组(>5)。免疫组织化学观察切片组织波形蛋白(Vimentin)表达情况,分析瘤芽数量与上皮间质转化的相关性以及与临床病理特征的关系,Kaplan-Meier法进行生存分析,组间差异采用Log-rank检验。结果:低瘤芽组共22例,高瘤芽组共18例,病理学检查发现瘤芽内肿瘤细胞形态与肿瘤主体细胞无差异,瘤芽数量与患者年龄、性别、无相关性,瘤芽与肿瘤大小、临床分期以及淋巴结转移呈正相关(P<0.05),高瘤芽组其Vimentin的表达率明显高于低瘤芽组,提示瘤芽与口腔癌EMT过程相关,是EMT的形态学表现。两组患者的中位生存期(overallsurvival,OS)分别为40.4,26.5个月(χ2=5.265,P=0.012),两组中位无进展生存期(progression-freesurvival,PFS)分别为35.8,22.1个月(χ2=6.744,P=0.009)。结论:口腔鳞状细胞癌组织瘤芽数量与EMT密切相关,代表恶性和侵袭能力更高的癌细胞亚群,可作为口腔鳞状细胞癌患者的预后判断指标。
Objective: To investigate the correlation between tumor budding and epithelial-mesenchymal transition(EMT), further explore the relationship with clinical-pathological features and clinical prognosis. Methods: To collect 40 resected specimens from patients with oral squamous cell carcinoma who were admitted in our hospital from 2007 to 2013. They were divided into 2 groups according to dif erent number of tumor buddings by HE staining, which were low tumor buddings(≤5) and high tumor buddings(>5). Expression of Vimentin in specimens was explored by immunohistochemistry. The relationship between tumor budding and EMT and clinical-pathological features was analyzed. Estimation of overall survival(OS) and progression free survival(PFS) distributions was performed by Kaplan-Meier method and differents between groups were determined by log-rank tests. Results: Low tumor budding group(n=22) and high tumor budding group(n=18) by HE staining revealed no signii cant different between intratumoral bud cells and cells in tumor body. No correlation between tumor budding number and ages and gender of patients were investigated. Statistical analysis revealed that tumor budding was associated with tumor size, lymph node metastasis and distant metastasis, clinical stage(P<0.05). In addition, significant association was observed between tumor budding and up-regulation of Vimentin(P=0.001), revealing that tumor budding is associated with EMT of OSCC and morphological feature of EMT. The median overall survival time of two groups was 40.4, 26.5 months respectively(χ2=5.265, P=0.012). The median progression free survival time of two groups was 35.8, 22.1 months respectively(χ2=6.744, P=0.009). Conclusion: Tumor budding, which is associated with EMT, represents worse malignant and invasive cancer cells subpopulation. It could be an independent prognostic factor in OSCC.
出处
《临床与病理杂志》
2015年第10期1788-1793,共6页
Journal of Clinical and Pathological Research
基金
东莞市医疗卫生科技计划一般项目(2015105101057)~~
关键词
口腔鳞状细胞癌
上皮–间质转化
瘤芽
波形蛋白
预后分析
oral squamous cell carcinoma(OSCC)
epithelia-mesenchymal transition(EMT)
tumor budding
vimentin
prognostic analysis
