摘要
目的 探讨人胎盘贴壁细胞 (hPDAC)的分离培养及造血相关因子的表达。方法 酶消化法分离培养人胎盘组织中贴壁细胞 ,并鉴定其生物学特性 ,用RT PCR方法检测造血相关因子表达。结果 成功分离培养出hPDAC ,并证实其具有间充质干细胞特性 ,表达多种造血相关因子 ,包括SCF、FL、G CSF、GM CSF、M CSF和IL 6。结论 hPDAC可为脐血CD34+ 细胞体外扩增提供潜在滋养层。
Objective To isolate and culture human placenta derived adherent cells (hPDAC) and assay their hematopoietic growth factor expression. Methods By enzyme digestion, hPDAC were isolated from human placenta tissue and cultured, and their biological characteristics were studied. The hematopoietic growth factor (HGF) mRNA expression of hPDAC was assayed by RT-PCR.Results hPDAC was successfully isolated from human placenta tissue, which was further confirmed as mesenchymal stem cell-like cells. HGF including SCF, FL, G-CSF, GM-CSF, M-CSF and IL-6 were expressed in hPDAC. Conclusion hPDAC could be used as feeder layer for umbilical cord blood CD 34+ cells ex vivo expansion.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2003年第12期652-654,共3页
Chinese Journal of Hematology
基金
国家自然科学基金资助项目 ( 3 0 2 712 45 )
关键词
人胎盘贴壁细胞
分离培养
造血相关因子
表达
Human placenta derived adherent cells
Isolation and culture
Hematopoietic growth factors
