摘要
【目的】探索骨髓基质细胞抗原2(bone marrow stromal cell antigen 2,Bst2)对颅脑创伤的影响。【方法】将大鼠随机分为假手术组,创伤性颅脑损伤(Traumatic brain injury,TBI)组,TBI+Bst2 siRNA组。其中TBI+Bst2 siRNA组在损伤后同时原位注射Bst2 siRNA。通过改良大鼠神经功能缺损评分,伊文思蓝通透性,脑干湿比重比较Bst2敲低对TBI损伤大鼠的影响。通过免疫印迹检测水通道蛋白-4(aquaporin 4,AQP4),基质金属蛋白酶-9(matrix metalloprotein,MMP9),组织型纤溶酶原激活物(tissue plasminogen activator,tPA),肿瘤坏死因子-α(tumor necrosis factor-α,Tnf-α),表皮生长因子受体(epidermal growth factor receptor,Egfr)表达水平的变化。【结果】Bst2 siRNA降低了TBI大鼠神经功能损伤评分,降低了伊文思蓝通透性,降低了脑水肿;免疫印迹结果显示Bst2敲低后Aqp4,Mmp9,tPA,Tnf-α和Egfr的表达水平降低。【结论】Bst2敲低通过降低Aqp4,Mmp9,tPA,Tnf-α和Egfr的蛋白水平减轻了脑水肿,改善了血脑屏障通透性和神经功能。
【Objective】To explore the effects of bone marrow stromal cell antigen 2(Bst2)on the traumatic brain injury(TBI)in rats.【Methods】The rats were randomly divided into sham group,TBI group and TBI+Bst2 siRNA group.The rats in the TBI+Bst2 siRNA group were injected with Bst2 siRNA at the in-situ injury area after TBI.Modified neurological severity score,(mNSS),Evans blue(EB)dye extravasation and water content of the brain were used to explore the effect of Bst2 knockdown on TBI rats.The levels of Aquaporin4(Aqp4),matrix metalloprotein 9(Mmp9),tissue plasminogen activator(tPA),tumor necrosis factor-α(Tnf-α),epidermal growth factor receptor(Egfr)were assessed by immunoblot.【Results】The siRNA of Bst2 reduced the mNSS,EB dye extravasation and water content of the brain after TBI.Furthermore,the siRNA of Bst2 also reduced the levels of Aqp4,Mmp9,tPA,Tnf-αand Egfr after TBI.【Conclusion】Bst2 knockdown improves the brain edema,permeability of blood-brain barrier and neurological functions by reducing the protein levels of Aqp4,Mmp9,tPA,Tnf-αand Egfr.
作者
胡艇
张赛
HU Ting;ZHANG Sai(Tianjin Key Laboratory of Neurotrauma Repair,Institute of Traumatic Brain Injury and Neuroscience,Center for Neurology and Neurosurgery,Characteristic Medical Center of PAP,Tianjin 300162,China)
出处
《武警后勤学院学报（医学版）》
CAS
2019年第3期18-21,34,共5页
Journal of Logistics University of PAP（Medical Sciences）
关键词
骨髓基质细胞抗原2
创伤性颅脑损伤
脑保护
脑水肿
血脑屏障
Bone marrow stromal cell antigen 2
Traumatic brain injury
Cerebral protection
Brain edema
Blood–brain barrier
