摘要
目的探讨游泳对妊娠和哺乳期暴露于阿特拉津(atrazine,ATR)的大鼠雄性子代中脑多巴胺(DA)能神经元(dopaminergic neuron)损伤改善的可能性机制。方法 60只雌雄SD大鼠2∶1交配至雌鼠怀孕,在妊娠第1天(GD1)随机分为2组:对照组(n=8)和ATR染毒组(n=24,100 mg/kg·bw ATR),从GD 5至出生后第21天(PND 21)每周灌胃染毒2次。直到子鼠1.5个月龄,将染毒组子鼠随机分为:ATR暴露组,ATR+8 d训练组和ATR+28 d训练组。待子鼠3月龄,抓力测试大鼠运动协调力变化,取中脑观察神经细胞变化情况并检测Nurr1、TH和c-fos表达水平,纹状体检测DA含量变化及脑组织病理学检验。结果 ATR暴露组与对照组相比,大鼠抓力、纹状体DA含量以及中脑内Nurr1、TH和c-fos明显降低(P<0.05);ATR+8 d训练组与ATR暴露组相比,除TH基因升高(P<0.05),其他均无统计学意义;而ATR+28 d训练组与ATR暴露组相比,除c-fos基因变化无统计学意义,其他都明显上调(P<0.05)。病理结果显示ATR暴露组可引起中脑神经细胞病理学损伤,训练组可改善这种情况。结论游泳可改善由生命早期ATR暴露所致的大鼠中脑DA能神经毒性,并揭示了运动训练有助于改善DA能神经元损伤的能力。
Objective To explore the possible mechanism of swimming on the improvement of the midbrain dopaminergic neuron injury in male rats exposed to atrazine(ATR)during gestation and lactation.Methods Sixty female and male Sprague-Dawley rats were mated at 2∶1 and randomly divided into two groups on the gestational day 1(GD1):control group(n=8)and ATR-treated group(n=24,100 mg/kg·bw ATR),intragastrically administered twice a week from GD 5 to postnatal day 21(PND 21).Until offspring 1.5 months of age,the pups were randomly divided into ATR exposure group,ATR+8 day training group and ATR+28 day training group.At 3 months of age,motor coordination of the pups and midbrain neurons pathological changes were tested.The expression levels of Nurr1,TH and c-fos in the midbrain and the content of DA in the striatum were detected.Result The grip strength,striatal dopamine content and Nurr1,TH and c-fos in the midbrain were significantly lower in the ATR exposed group than in the control group(P<0.05).Compared with the ATR exposure group,the ATR+8-day training group had no statistical significance except for the increase of TH gene(P<0.05),while the ATR+28-day training group was significantly up-regulated except for no statistically significant change in c-fos gene.HE result showed that the ATR exposed group caused neuronal pathological damage,and the training groups could improve this condition.Conclusion Swimming improves the midbrain dopaminergic neurotoxicity induced by early life ATR exposure and reveals the ability of exercise training to improve dopaminergic neuronal damage.
作者
王丹丹
李柏
吴艳萍
李百祥
WANG Dan-dan;Li Bai;WU Yan-ping;LI Bai-xiang(Institute of Toxicology, College of Public Health, Harbin Medical University, Harbin Heilongjiang 150081,China)
出处
《毒理学杂志》
CAS
CSCD
2019年第2期102-107,共6页
Journal of Toxicology
